Yeast Fermentation Process for Glycolipid Production

Glycolipids are lipids, consisting of a combination of sugars and lipids. Glycolipids can be used as biosurfactants. Compared with chemically produced surfactants, glycolipids have more advantages, such as lower toxicity, better biodegradability, environmental compatibility, and higher foaming capacity. Yeast is a unicellular organism with relatively simple genetic manipulation and is free of endotoxin. It is widely used in various biotechnologies, such as ethanol and glycolipid production. Currently, saccharolipids can be produced by the yeast fermentation process. As one of the glycolipid bioactive agents, saccharolipids are produced by Candida apicola. In order to obtain a more efficient yeast fermentation process for glycolipids, it can be optimized in three ways. Firstly, the process can be optimized in terms of medium, culture conditions, and product recovery. Secondly, we try to use industrial wastes and cheap substrates. Finally, we maximize the yield by mutagenesis or recombination of high-yielding bacteria.

Our Quality Services

CD BioGlyco's yeast fermentation process and glycolipid production technology is well established. We provide a one-stop shop for glycolipids. Our yeast fermentation process services include but are not limited to the following.

• Yeast fermentation process service
  Saccharolipids are microbial secondary metabolites produced by pseudohyphae (Candida apicola) through a fermentation process under certain conditions, using sugar and vegetable oils as carbon sources. The fermentation process includes strain cultivation, fermentation culture, glycolipid extraction, purification, and product quality testing. Our sophorolipid yeast fermentation process consists of a total of four types:

Flow-addition fermentation process
It is one of the most commonly used fermentation processes in saccharolipid production, which is based on the flow addition of carbon sources, and the saccharolipid yield is significantly increased.

Semi-continuous fermentation process
The development of a semi-continuous fermentation process can effectively shorten the auxiliary time required in the batch-switching process, and reduce the production cost of saccharolipid. At the same time, it can reduce the inhibitory effect of the product to a certain extent and alleviate the foaming problem during the fermentation process.

Continuous fermentation process
Yeast cells are retained in the reactor, while the product can be separated with the fermentation effluent. The product inhibition effect is reduced to a certain extent and the accumulation of harmful metabolic wastes is avoided.

Solid-state fermentation process.
Compared with the traditional liquid fermentation, it can avoid the foam problem, cut can use the waste substrate as the substrate.

• Process optimization service
  The fermentation process of sophorolipid uses glucose as the carbon source and yeast powder or corn syrup as the nitrogen source. In order to reduce the production cost of sophorolipid, we are committed to using industrial and agricultural waste (molasses, straw, bagasse, waste oil, food waste, etc.) as the carbon source. At the same time, we optimize fermentation process parameters, including medium composition (nitrogen source, metal ions, carbon source), fermentation process parameters (pH, temperature), and physiological parameters (biomass, dissolved oxygen). We also compared the effects of different separation and purification methods on the purity of sophorolipid, including gravity separation, froth flotation, solvent extraction, chromatography, low-temperature crystallization, membrane filtration, etc.

Fig.1 Manufacture process of sophorolipid. (CD BioGlyco)

• Sophorolipid production service (sophorolipid annual output of 50,000 tons)
  As a glycolipid biosurfactant, sophorolipid has excellent surface activity and has been commercialized by many companies. Our fermentation process is entirely a microbial metabolism process and the raw materials are of natural plant origin. Our extraction process is a physical extraction process, including natural sedimentation, centrifugation, and filtration, without organic solvents. All processes are free of chemical reactions and chemical additives, ensuring that the overall process is environmentally friendly.

Applications

• As a natural surfactant, glycolipid can be used as a substitute for synthetic surfactants in the manufacture of various daily detergents. It has better foaming and biodegradability.
• Glycolipid can be used as a detergent for cleaning soil and sewage pipes. It can also be used in a variety of daily detergents such as soaps and cleansers.
• Glycolipid synthesis technology also has an important role in agriculture. Synthesized glycolipids can promote plant nutrient uptake and improve the utilization of pesticides and waste materials.

Advantages

• We use an environmentally friendly fermentation process. Our glycolipid is very pure and available in a variety of sizes. No chemicals are added to the product.
• Fermentation-synthesized glycolipid has a variety of high-quality properties and a wider range of applications.
• The technology of glycolipid fermentation synthesis helps researchers explore the value of its application in the pharmaceutical industry.
• Glycolipid synthesized through fermentation has a similar detergent effect compared to synthetic detergents. Glycolipids can replace synthetic detergents to solve the problem of eutrophication of water bodies.

CD BioGlyco provides high-quality glycolipids, producing up to 50,000 tons of glycolipids per year through a yeast fermentation process. We also provide a Bacterial Fermentation Process-based Glycolipid Manufacture Service and Chemical Post-Treatment-based Glycolipid Manufacture Service. If you want to get more detailed information, you can contact us and we will answer you one by one.

• Bacterial Fermentation Process-based Glycolipid Manufacture Service
• Chemical Post-Treatment-based Glycolipid Manufacture Service
• Enzymatic Post-Treatment-based Glycolipid Manufacture Service