Structure of Paramylon

Paramylon is a liner beta-(1,3)-glucan with a structure similar to Curdlan. It is deposited in the cells of euglenoids in the form of microscopic granules, especially in microalga Euglena gracilis, which is a highly robust microorganism capable of surviving in various nutritional conditions. Paramylon is neither water-soluble nor thermoplastic due to its crystalline structure, depending on the stable triple helix formed by intramolecular hydrogen bonding.

Fig.1 Multiscale structure of native paramylon. (Feuzing, et al., 2022)

Advantages of Paramylon

Paramylon can be recognized and taken up by specific receptors on the surface of macrophages, activating non-specific immunity in the human body. It can act as a dietary fiber to regulate intestinal flora and protect intestinal health and support intestinal immune function. Paramylon is not absorbed by the body, and its molecular interior has a porous structure, with the ability to absorb excess cholesterol, neutral fat, heavy metals, alcohol, etc., and discharge them from the body. Therefore, it has a strong effect on antioxidation, antivirus, and free radical scavenging. In addition, paramylon has a unique role in protecting the liver, alleviating atopic dermatitis, inhibiting purine absorption, and improving ventilation.

Fig.2 Immune activation of the paramylon. (Guo, et al., 2020)

Paramylon Manufacture Service

Paramylon has special advantages in food and pharmaceutical industries, worthy of further research and application. CD BioGlyco has the best paramylon manufacture platform to provide customers with paramylon in high purity and good activity.

• Microorganism cultivation: CD BioGlyco cultures the non-chloroplastic WZSL mutant of Euglena gracilis to obtain linear beta-(1,3)-linked glucans with 100% purity.

Fig.3 Paramylon granules extracted from WZSL mutant of Euglena gracilis. (Barsanti & Gualtieri, 2019)

• Paramylon extraction: Ultrasound-assisted extraction and enzymolysis are common methods. CD BioGlyco designs and provides the optimal process conditions for paramylon extraction.
• Purification technologies: Diethylaminoethyl cellulose column chromatography and dextran gel column chromatography are widely used in paramylon purification.
• Purity identification: CD BioGlyco has different physicochemical methods for purity identification of paramylon, such as the phenol-sulfuric acid process, the ultraviolet spectrum analysis, infrared spectroscopic analysis, etc.
• Determination of molecular weight: Gel permeation chromatography is commonly used.
• Analysis of monosaccharide composition: Technologies including ¹H nuclear magnetic resonance (¹H NMR), ¹³C NMR, infrared spectroscopy, gel permeation chromatography, etc. are usually applied.

You can see more comprehensive beta-glucan analysis methods and techniques in our Beta-Glucan Quantification Service and Beta-Glucan Structural Analysis Service.

Applications

• Research in thermoplastic films
• Raw materials of optical lenses
• Nanofibers with a specific self-assembly mechanism
• Functional food
• Pharmaceutical research

Advantages

• Paramylon with high purity: up to 99%
• The optimal extraction process for paramylon
• Comprehensive characterization of the product

CD BioGlyco has unique advantages in the production process of paramylon to provide customers with satisfactory service quality. If you are interested in our services, please contact us for more information.

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