Enzymatic extraction is a modern biotechnological tool that utilizes the catalytic activity of enzymes to achieve high-efficiency extraction and purification of molecules. This method has many advantages such as high productivity, specificity as well as environmental friendliness. In addition, enzymatic extraction is gentler and more efficient than traditional methods, either physical or chemical, and prevents the destruction of molecule structures and activities. The successful applications of enzymatically extracted products involve food, medicine, and bioengineering fields. In food manufacturing, enzymatically extracted polysaccharides yield naturally occurring gum polysaccharides, which are utilized for functions such as thickening, gelling, and stabilizing emulsions. In the field of pharmaceuticals, they are used in preparing high-purity polysaccharide drugs with immunomodulatory, anti-tumor, and antibacterial activities. In the field of bioengineering, enzymatically extracted polysaccharides can be used to produce polysaccharide-related bioproducts, such as biological collagen and polysaccharide carriers.
As one of the leading companies in the field of Polysaccharide Manufacturing and analysis, CD BioGlyco has many years of experience in the enzymatic extraction of polysaccharides and offers customized services according to your needs. The detail of our service is as follows:
According to the type and structure of the target molecule, choose the suitable enzyme species and substrate. Different sugar classes correspond to different enzymes, e.g. mylase is suitable for starch degradation, cellulase is suitable for cellulose degradation, and so on. Pre-treatment of the raw material, including crushing, alkali treatment, acid treatment, or heat treatment. This disrupts the cell wall, reduces the influence of non-polysaccharide components, and makes it easier for enzymes to access and act on the target molecule.
The enzyme and substrate are added to the reaction system under specific temperature, pH, and time conditions to ensure optimal enzyme activity. The enzymatic reaction is stopped by changing the reaction conditions or by adding a reaction-stopping agent to prevent further target molecule degradation or loss of activity.
Isolation and purification of products and analysis and evaluation of extracted products to determine the purity, molecular weight, structure, and other characteristics.
Fig.1 Process of enzymatic extraction. (CD BioGlyco)
CD BioGlyco has years of experience in polysaccharide extraction, and we are committed to providing high-quality enzymatic extraction services and related troubleshooting solutions. We look forward to being your best partner in your research. If you are interested in our services, please
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