Necessity of Oligosaccharide Separation

Carbohydrates have long been considered an important component of biological structure and energy sources. Oligosaccharides are distributed on the surface of cells and are often strongly related to biological functions such as cell interaction, recognition, and defense. The development of oligosaccharides in food, medicine, and agriculture has great potential, while the separation of high-purity oligosaccharides is the prerequisite for studying their unique structures and remarkable biological activities.

Difficulties in Oligosaccharide Separation

High-purity oligosaccharide separation has always been the focus and difficulty in industrial production. First, oligosaccharides have a similar chemical composition and complex structures, with many possible isomers and kinds of connection and branching forms. Second, the absence of chromogenic groups or fluorophores on oligosaccharide molecules makes it difficult to detect, and the content of oligosaccharide components in physiologically active glycoconjugates may be small. Over the years, Chromatographic technology has been rapidly developed and generally used in the separation and purification of oligosaccharides, which has brought the dawn of small quantity and even high throughput preparation of high-purity oligosaccharides.

Our Services

CD BioGlyco has abundant experience in oligosaccharide separation. We provide our customers with various efficient chromatographic separation technologies to ensure that you get 100% pure oligosaccharide products.

Fig.1 Different chromatographic technologies for oligosaccharide separation. (CD BioGlyco)

• High-performance anion exchange chromatography (HPAEC)

Sugars are polyhydroxy aldehydes or ketones, and exist as anions at a high pH value and can be retained in the column. The stationary phase of HPAEC is a highly efficient anion exchange resin with high strength and stability. HPAEC combined with pulsed amperometric detection (PAD) is an effective chromatographic method for oligosaccharide analysis.

• Graphitized carbon liquid chromatography (GCLC)

The stationary phase of the chromatography is porous graphitized carbon filler, which has a uniform surface structure and good stability, and is suitable for a wide pH range (pH 1~14). Chromatography technology has been commonly used in the separation of various oligosaccharides.

• Hydrophilic interaction liquid chromatography (HILIC)

HILIC is an extension of forward chromatography in the field of water-soluble mobile phase, which is usually water (less than 40%) and organic solvent (like acetonitrile), and the stationary phase is various hydrophilic polar materials with special characteristics, which can be selected according to the different properties of the target oligosaccharides.

• Capillary electrophoresis (CE)

Once oligosaccharide molecules are electrically charged in an appropriate buffer or derivatized, CE is an efficient technique to separate these oligosaccharides. Separating glycoconjugates (glycoproteins, glycolipids, proteoglycans) by CE is a good choice.

• Lectin affinity chromatography (LAC)

Lectin is a kind of protein that can specifically bind to sugar molecules, which can be fixed on the stationary phase to achieve efficient separation of sugars. LAC possesses many advantages such as high selectivity, simple operation, and wide application.

Applications

• Chemical structure characterization of oligosaccharides
• Small quantity or high throughput production of high-purity oligosaccharides
• Glycobiology developmental

Highlights

• A variety of advanced chromatographic separation technologies
• Industrial preparation capacity of high-purity oligosaccharides
• Professional technical personnel and scientific process
• End-to-end service

As a reliable and goal-oriented carbohydrate supplier, CD BioGlyco offers customers a wide range of science-backed products and customized solutions, aimed at supporting your entire product life cycle. It takes more than Monosaccharide, Oligosaccharide, or Polysaccharide products, it takes a partner. CD BioGlyco provides you with end-to-end service. Please feel free to contact us.

• Simulated Moving Bed (SMB) Chromatography for High-Purity Oligosaccharide Manufacture
• Microbial Purification/Selective Fermentation for High-Purity Oligosaccharide Manufacture