The Role of SEC Analysis

SEC is separated according to sample hydrodynamic volume, and characterization of polysaccharides according to their structural parameters (size, molar mass, distribution, and structure). The molecular sieve is utilized to enable molecules of different sizes and numbers to complete the separation of components according to the different exclusion capabilities of each component. It is the key to a better understanding of structure-property relationships, the ability to determine the true molar mass, the structure of the macromolecules, and the composition of the copolymer detectors coupled to chromatographic systems. The rheological properties and solubility of feedstocks depend on their molar mass and branching, so good characterization along the process is important. SEC analysis is well established for the determination of oligosaccharides and medium-sized polysaccharides.

Fig.1 The separation of SEC. (Lubomirsky, et al., 2021)

DP/MW Analysis by SEC Services at CD BioGlyco

Molecular properties such as weight average molecular mass (MW), average radius of rotation, and intrinsic viscosity of soluble dextran are determined by SEC, polysaccharides are commonly dissolved in the aqueous system or analyzed directly in the extracts. CD BioGlyco provides the latest analysis services to improve the size separation range and sensitivity of target analytes. We have a variety of detectors including light scattering, fluorescence, refractive index (RI), viscometer, and mass spectrometry for SEC analyses. These detectors in keeping with traditional UV-visible spectrophotometer detectors, greatly expand their ability to characterize the primary and higher-order structures.

At CD BioGlyco, we offer several high-resolution and high-sensitivity methods including mass spectrometry-based methods, high-performance liquid chromatography (HPLC), etc. Combined with high-throughput technologies, advanced instrumentation, and state-of-the-art data processing capabilities, we assist our customers in structural characterization, speed up the progress of the project, and provide the most comprehensive data analysis report.

Fig.2 SEC in-line dection method for beta-glucan. (CD BioGlyco, 2023)

Applications

• DP/MW analysis by SEC is used to integrate separation and characterization.
• DP/MW analysis by SEC is suitable for drug molecular screening.
• Characterization of macromolecular chain separation by SEC of DP/MW analysis.
• DP/MW analysis by SEC accelerates the study of the structure-effect relationship.

CD BioGlyco has always adhered to the customer-centric principle and developed a set of solutions for Beta Glucan Structural Analysis. We are capable of providing efficient and reliable services to accelerate our clients' scientific research by shortening project time. Moreover, we provide Beta-Glucan Manufacture, Quantification, Fermentation Profiling, or Vaccine Adjuvants Development services. If you need our services, please feel free to contact us, and we will reply as soon as possible.

• DB Analysis
• Linkage Ratio Analysis