Advantages of Physical-/Chemical Process for Oligosaccharide Manufacture

Oligosaccharides are ubiquitous molecules in life. Oligosaccharides are low molecular weight carbohydrates that, when indigestible, produce bifid activity and other effects on human health. Oligosaccharides occur naturally in foods or are synthesized from disaccharide substrates, or produced by the hydrolysis of polysaccharides. Although there is room for improvement, this method is the best choice for large-scale production of oligosaccharides due to its reproducibility. For polysaccharides such as cellulose that are difficult to hydrolyze due to structural entanglement, using supercritical treatment to hydrolyze them has a better effect. During this process, cellulose is converted into water-soluble cellobiose, cellotriose, and cellulose pentose.

Chemical synthesis is an important method of exploratory research, as this well-established discipline provides access to almost any structure. This makes chemical synthesis extremely important in fields such as medicine that require well-defined oligosaccharide structures.

Fig 1 Synthesis of Globo H and stage-specific embryonic antigen. (Krasnova & Wong, 2019)

Physical-/Chemical Process for Oligosaccharide Manufacture at CD BioGlyco

Oligosaccharide Manufacture is one of the key services we have been focusing on. CD BioGlyco offers advanced Technologies for Oligosaccharide Manufacture. Among these technologies, the physical-/chemical process for oligosaccharide manufacture is an important type of oligosaccharide production technology that we provide. The technical services we provide include but are not limited to the following.

Supercritical Treatment

Hydrothermal techniques are particularly suitable for obtaining oligosaccharides from lignocellulosic biomass. These techniques are used at high temperatures and pressure, with or without catalysts. Hydrothermal technology mainly includes autohydrolysis, subcritical hydrolysis, supercritical hydrolysis, and so on.

Chemical Hydrolysis

Physicochemical or simple hydrolytic treatment with the addition of a small amount of acid catalyst is an efficient treatment for hemicellulose hydrolysis and partial hydrolysis of cellulose, resulting in wood oligomers and fiber oligomers. Correct conditions (temperature, residence time, acid load, biomass load) are essential to obtain a satisfactory yield of oligosaccharides from biomass feedstock.

Chemical Synthesis

Although this is not the main technology for oligosaccharide production, chemically synthesized homogeneous oligosaccharides are still favored in fields such as oligosaccharide pharmaceuticals. CD BioGlyco provides strategies such as metal-catalyzed glycosylation reactions, non-metal-catalyzed glycosylation reactions, one-pot glycosylation reactions, and solid-phase glycosylation reactions for the chemical synthesis of oligosaccharides.

Applications

• Glycan library creation: The physical-chemical process for oligosaccharide manufacture is extensively utilized in creating diverse glycan libraries. These libraries serve as invaluable resources for deciphering the roles of various glycans in biological interactions. Researchers can synthesize structurally varied oligosaccharides, enabling comprehensive studies into glycan-protein interactions, molecular recognition, and potential therapeutic targets.
• Biopharmaceutical development: This method finds application in the production of oligosaccharides with specific structural features required for biopharmaceutical development. Glycosylation plays a pivotal role in modulating the efficacy, stability, and immunogenicity of biologics. By precisely tailoring oligosaccharide structures through physical-chemical processes, biopharmaceuticals with enhanced therapeutic properties and reduced side effects can be engineered.
• Functional food production: Oligosaccharides with specific prebiotic properties are in high demand for functional food development. Physical-chemical processes enable the controlled synthesis of oligosaccharides with desired degrees of polymerization and functional groups. These oligosaccharides can serve as prebiotics, selectively nourishing beneficial gut microorganisms and contributing to digestive health when incorporated into food products.

Advantages

• Advances in the physical-/chemical process contribute to an improved understanding of glycosylation.
• The physical-/chemical process is used as an important supplement technology to the enzymatic production of oligosaccharides, making it possible to produce more types of oligosaccharides.
• The more well-defined oligosaccharides produced by the physical-/chemical process are helpful for drug development.

CD BioGlyco provides an integrated One-Stop Solution for Carbohydrate Manufacture. Our advanced physical-/chemical process for oligosaccharide manufacture and high-quality service will fully meet your needs. If you are interested in our services, please contact us for more details without any hesitation.

• Enzymatic Process for Oligosaccharide Manufacture
• Chemoenzymatic Process for Oligosaccharide Manufacture
• Separation Process for High-Purity Oligosaccharide Manufacture
• Supercritical Treatment
• Chemical Hydrolysis
• Chemical Synthesis