Advantages of Enzymatic Synthesis of Glycolipid (GL)

GL is a very versatile class of ecological biosurfactants. It has good foaming and also exhibits some antimicrobial activity. This makes GL a suitable candidate for cosmetic, pharmaceutical, and biocontrol applications. There are fewer types of GLs synthesized by conventional fermentation. Researchers have now developed enzyme-catalyzed synthesis of GL. Enzymatic synthesis of GLs can generate a wide variety of new GLs, with a diversity that far exceeds that of natural GLs.

Enzymatic methods have the advantages of regioselectivity, substrate diversity, and reusable catalysts, while enzymatic methods also reduce the generation of by-products. In contrast to chemical synthesis, pure GLs can be obtained without the use of protecting groups. The enzymatic process is in line with the principles of green chemistry, especially if the solvent system is properly selected. The synthesis of GL by lipase catalysis in organic solvents is a common method. β-glucosidase has also been developed as a lipase alternative for GL synthesis.

Fig.1 Benefits of the enzymatic method. (CD BioGlyco)

Services

As a high-tech bio-enterprise, CD BioGlyco specializes in the research, production, and marketing of GL derivatives. We provide enzymatic synthesis services for customized GLs.

• GL synthesis services

Under enzyme-catalyzed conditions, almost all sugar and fatty acid derivatives can be synthesized into a rich variety of GL surfactants. We are equipped with a specialized research team to develop new GLs by enzymatic synthesis. lipase and β-glucosidase are highly regioselective. they can form ester bonds and highly stable ether bonds. We mainly catalyze the synthesis of GLs by lipase and β-glucosidase. Studies have shown that the novel GLs generated are of low toxicity and have biological activities such as antimicrobial, antiviral, antitumor activities, etc.

The solubility of glycolipid donors in the same medium is a constraint for GL synthesis. The enzymatic synthesis process also requires the maintenance of enzyme activity and biosafety, so the selection of a suitable reaction solvent is needed. Utilizing renewable resources as reaction substrates, we screen the most suitable solvent system and reaction conditions to improve the safety of the reaction.

We are also equipped with various testing instruments such as high-performance liquid chromatographs, gas chromatographs, polarimeters, etc. All products are strictly monitored by quality control to ensure quality.

Fig.2 Process study of enzyme-modified GL synthesis. (CD BioGlyco)

Lipase Catalyzed Glycolipid Synthesis

Lipases catalyze the synthesis of various compounds by linking the sugar portion to alkyl residues via ester bonds. CD BioGlyco screens for optimal reactants and conditions for lipase-catalyzed GL synthesis based on extensive experience in glycochemistry.

β-Glucosidase Catalyzed Glycolipid Synthesis

β-Glucosidase is inexpensive to purchase, acts on a wide range of substrates, and has also been used to catalyze GL synthesis. CD BioGlyco leverages the strong technical capabilities of its own R&D department to provide customized GL services.

• Glycolipid products

We have a wide range of glycolipid products, including psychosines, digalactosylceramide, diglycosyl ceramides, dipalmitin, and so on. Product quality is guaranteed.

Applications

• Enzymatic modification techniques allow the synthesis of structurally different GLs, enabling researchers to study the functional relationships between different bond types (ester/ether).
• Enzymatic modification techniques have an important role in pharmaceuticals. Modified GLs can be used for drug development.
• Enzymatic modification technology is used to develop more effective soil remediation agents, cleaning agents, etc.

Advantages

• We specialize in esterification reactions, saccharification reactions, etc., and are adept at synthesizing GLs using various enzymes.
• Our assay for GLs is less restrictive and our assay equipment is highly sensitive.
• Our glycolipid products have good detergency, foaming properties and biodegradability.

CD BioGlyco has always been committed to improving its GL manufacturing and R&D capabilities in order to provide our customers with high-quality and consistent products. In addition to GL, we also synthesize Carbohydrate Derivatives and Oligosaccharides by enzymatic process. If you have any questions about the glycolipid synthesis process, please feel free to contact us. We will answer your questions as soon as possible!

• Microbial Fermentation-based Glycolipid Manufacture Service
• Chemical Synthesis-based Glycolipid Manufacture Service
• Lipase Catalyzed Glycolipid Synthesis
• β-Glucosidase Catalyzed Glycolipid Synthesis