Functions of Beta-Glucan Fermentation

Carbohydrate-based fermentation engineering has become a hot topic of research in many fields as research on the physiological activities of polysaccharides has intensified. β-Glucan is a carbohydrate polymer found mainly in various cereals and fungi. β-glucan is fermented by the microbiota to short-chain fatty acids, mainly acetic, propionic, and butyric acids. In this case, about 100 high-throughput compounds are always the main metabolites: amino acids, nucleotides, and metabolic intermediates. These intermediates usually originate from glycolysis, the pentose phosphate pathway, and the tricarboxylic acid cycle. These metabolites may be involved in crosstalk between the host and its microorganisms. Numerous studies have demonstrated the effectiveness of β-glucan fermentation products in a variety of diseases and disorders, reportedly lowering serum cholesterol, glucose, and lipid profiles, modulating intestinal flora as well as maintaining body weight and also reducing the probability of rectal cancer. 

Beta-Glucan Fermentation Profiling Service at CD BioGlyco

Fermentation analysis is a developing high-throughput technique. CD BioGlyco has the ability to comprehensively analyze small molecules in cells, tissues, or whole organisms. Furthermore, we use a method for the identification of small metabolites by nuclear magnetic resonance coupled chromatography and mass spectrometry.

• We perform in vitro fermentation, untargeted metabolomics combined with 16S rRNA sequencing to clarify β-glucan fermentation.
• We provide molecular characterization, infrared spectroscopy, monosaccharide composition, methylation, 1D, and 2D-NMR analysis services. Then, scanning electron microscopy display to systematically assess catabolic processes in a model of β-glucan fermentation.

Elucidation of fermentation process metabolites through multiple methods to support your projects. These methods provide the basis for the physicochemical properties and physiological function of β-glucan while opening up the pathway for exploring dietary carbohydrate metabolism in digestion and fermentation, and for studying fermentation and microbiome analysis.

Fig.1 Flow chart of β-glucan fermentation analysis. (CD BioGlyco)

CD BioGlyco has extensive experience and proven analytical processes in polysaccharide fermentation. Equipped with our advanced instrumentation platforms and specialized knowledge of glycobiology, we provide fast, accurate, and comprehensive data analysis, laying the groundwork for you to build on your research path. If you are interested in our services, Beta-Glucan Manufacture, Quantification, or Vaccine Adjuvant Development, please do not hesitate to contact us.

• Beta-Glucan Manufacture Service
• Beta-Glucan Quantification Service
• Beta-Glucan Structural Analysis Service
• Beta-Glucan Vaccine Adjuvant Development