α-Amylase Treatment

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Properties of α-Amylase Treatment

According to the configuration, amylase is divided into α- or β- Amylase; α -amylase also known as liquefied amylase catalyzes starch hydrolysis to dextrin, systematically named 1,4-α-D-Glucan glucanohydrolase. When the enzyme acts on starch and glycogen, the resulting reduced sugar is α -type in the optical structure. It cuts off the glucosidic bonds of starch, glycogen, oligo, or glycan molecules, producing maltose, oligosaccharides, and α,1-4 glucose, which is one of the most widely used enzyme preparations in industrial production. While it exists in nature, the study of plant-derived α -amylase mainly focuses on the germination of rice, corn, barley, and soybean, where seeds need to rapidly hydrolysis stored starch as an energy source for growth. This enzyme uses Ca2+ as an essential factor and acts as a stabilizing factor and an activator, and some amylases are not Ca2+-dependent.

Mode action of the α-amylase. Fig.1 Mode action of the α-amylase. (CD BioGlyco)

α-Amylase Treatment Services

CD BioGlyco has a newly modified starch based on α-amylase, using corn starch, adding buffer solution and enzyme mix, magnetic stirring- inactivated after the reaction, finally, filtered and precipitated, washed several times with distilled water, and the product is dried and ground for further use. We use the Fourier transform infrared (FTIR), scanning electron microscope (SEM), x-ray diffraction (XRD), and differential scanning calorimetry (DSC) to determine the structure and index reaction generally in two stages.

  • First, amylose fast degradation oligosaccharides, which is the result of α-amylase in a random way on starch. At this stage the viscosity of amylose and its ability to react with iodine rapidly.
  • The reaction in the second phase is much slower than that in the first phase, including the slow hydrolysis of the oligosaccharides to generate the final products of glucose and maltose.

CD BioGlyco provides all kinds of Modified Starch Manufacture service, and the adsorbent is widely used in food, medicine, chemical industry, cosmetics, agriculture, and other related fields.

Preparation of porous starch flow of corn starch under α-Amylase action. Fig.2 Preparation of porous starch flow of corn starch under α-amylase action. (CD BioGlyco)

Applications

  • α-Amylase can be used to hydrolyze the starch into small particles in the fields of laundry and dish-washing detergents.
  • In the food industry, α-amylase can improve the shelf life and softness of baked goods.
  • α-Amylase is used in the saccharification or liquefaction of starch.
  • α-Amylase treatmed starch can improve the digestibility of animal feed.
  • α-Amylase is used in the production of thermoreversible starch gels.

Advantages

  • Real-time monitoring of the α-amylase treatment reaction process, and is an optimal process.
  • Testing of products for all indicators to ensure quality

CD BioGlyco has many methods available for Enzyme Modification analysis. We have a professional technical process and advanced instrumentation platform, which will provide you with the best service. If you are interested in our services, please feel free to contact us for more details.

References

  1. Zhang, B.; et al. Corn porous starch: Preparation, characterization and adsorption property[J]. International journal of biological macromolecules. 2011, 50(1): 250-256.
  2. Dura, A.; et al. Functionality of porous starch obtained by amylase or amyloglucosidase treatments[J]. Carbohydrate Polymers. 2014, 101: 837-845.
For research use only. Not intended for any clinical use.
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