Methods for the Production of Oligosaccharides

Nondigestible oligosaccharides are sourced from a wide range of natural origins, including various foods and other substances. In some instances, specific natural materials act as raw materials for oligosaccharide manufacturing. When oligosaccharides are naturally present in the raw material, an extraction process becomes necessary. Typically, this process involves dissolving the substrate in water, methanol, or other alcoholic mediums. Additionally, alternative techniques such as physical/chemical processes, enzymatic processes, and chemoenzymatic processes provide access to low molecular weight carbohydrates. Many of the prebiotic oligosaccharides used in current applications are derived through extraction from natural sources, enzymatic or acid hydrolysis of polysaccharides, or produced via enzymatic transglycosylation. Due to the complexity inherent in achieving high-quality oligosaccharides, it is crucial to maintain continuous research and development efforts aimed at refining existing methodologies and exploring novel approaches.

Fig 1 Oxidative method of polysaccharide depolymerization. (Amicucci, et al., 2020)

Technologies for Oligosaccharide Manufacture at CD BioGlyco

CD BioGlyco provides an integrated One-Stop Solution for Carbohydrate Manufacture. The solutions we provide include but are not limited to physical-/chemical process for oligosaccharide manufacture, enzymatic process for oligosaccharide manufacture, and chemoenzymatic process for oligosaccharide manufacture.

Physical-/Chemical Process for Oligosaccharide Manufacture

Physical/chemical processes mainly include Supercritical Treatment, Chemical Hydrolysis, and Chemical Synthesis. Hydrothermal techniques are particularly suitable for obtaining oligosaccharides from lignocellulose biomass. Simple hydrolysis treatment is an effective treatment method for cellulose hemihydrolysis and partial hydrolysis. Chemically synthesized homogeneous oligosaccharides are favored in fields such as oligosaccharide pharmaceuticals. At CD BioGlyco, an advanced physical-/chemical process for oligosaccharide manufacture and high-quality service will meet your needs.

Enzymatic Process for Oligosaccharide Manufacture

Enzymatic process involves the depolymerization of their more structurally related polysaccharides, such as cellulose and pectin. These processes occur due to the simultaneous action of different enzymes, mainly catalyzed by hydrolases and to a lesser extent by lyases. These enzymes release oligosaccharides by cleaving ether and ester linkages. At CD BioGlyco, we pay attention to every detail in the enzymatic process for oligosaccharide manufacture and strive for perfection in every process.

Chemoenzymatic Process for Oligosaccharide Manufacture

Chemoenzymatic methods play an important role in the synthesis of structurally precise oligosaccharides. By effectively blending the advantages of chemical and enzymatic synthesis, chemoenzymatic methods provide a powerful and versatile approach to oligosaccharide production. At CD BioGlyco, we pay attention to every detail in the chemoenzymatic process for oligosaccharide manufacture and strive for perfection in every process.

Using the technologies above, we provide Oligosaccharides with High Purity. The Oligosaccharides we provide include but are not limited to the following: Lactose, Trehalose, Kestose, Stachyose, Galactooligosaccharide, Maltooligosaccharide, Isomaltooligosaccharide, Fructooligosaccharide, Xylooligosaccharide, and Chitooligosaccharide.

Applications

• Technologies for oligosaccharide manufacture play an important role in the pharmaceutical industry, where the synthesis of specific oligosaccharides is crucial for drug development. Oligosaccharides with defined structures are used as vaccine components, contributing to the development of new treatments for various diseases.
• In the field of food nutrition, oligosaccharide manufacturing technology is used to produce functional oligosaccharides with potential health benefits.
• Oligosaccharide manufacturing techniques are an integral part of glycobiology research, allowing scientists to study the structure and functions of specific oligosaccharides in biological systems.
• Application of enzyme engineering techniques to improve the efficiency and specificity of chemoenzymatic methods in oligosaccharide synthesis.

Advantages

• Technologies for oligosaccharide manufacture allow the synthesis of a wide variety of structurally diverse oligosaccharides, enabling researchers to explore their diverse biological functions.
• Technologies for oligosaccharide manufacture facilitate the study of glycan structures and their roles in various biological processes, including cell signaling and immune responses.
• The ability to synthesize a wide variety of oligosaccharides facilitates in-depth studies of glycan-protein interactions, glycosylation processes, and their impact on various biological functions.

CD BioGlyco is a world-leading independent biotechnology company, focusing on high-quality technologies for oligosaccharide manufacture. If you are interested in our services, don't hesitate to contact us for comprehensive information.

• Lactose Manufacture Service
• Trehalose Manufacture Service
• Kestose Manufacture Service
• Stachyose Manufacture Service
• Galactooligosaccharide Manufacture Service
• Maltooligosaccharide Manufacture Service
• Isomaltooligosaccharide Manufacture Service
• Fructooligosaccharide Manufacture Service
• Xylooligosaccharide Manufacture Service
• Chitooligosaccharide Manufacture Service
• Physical-/Chemical Process for Oligosaccharide Manufacture
• Enzymatic Process for Oligosaccharide Manufacture
• Chemoenzymatic Process for Oligosaccharide Manufacture
• Separation Process for High-Purity Oligosaccharide Manufacture