Principle of Ion Exchange Column Chromatography (IECC)

IECC is a purifying method of charged substances based on the selective adsorption and elution of charged substances in solution by ion exchange (IE) resins. Polysaccharides are a class of biomolecules with complex structures and multiple ionic sites. IECC effectively separates and purifies polysaccharides by utilizing selective adsorption and elution features. Characterized by high selectivity and high resolution, IECC effectively separates compounds with different charge properties or ionicity. In addition, IECC is relatively simple to operate and is suitable for batch sample processing or large-scale production. Therefore, IECC is also an efficient technological tool that is extensively used in polysaccharide purification, providing an efficient and reliable purification strategy for research and application areas. Through IECC, high-purity polysaccharide samples can be obtained to provide strong support for further studies.

Fig.1 Types of ion exchangers. (Acikara, 2013)

IECC-based Polysaccharide Purification Service at CD Bioglyco

CD BioGlyco has accumulated abundant experience in customized Polysaccharide Manufacturing Services, especially in Starch Manufacturing and Glucan Manufacturing. We are also willing to provide necessary assistance for our clients in IECC-based Polysaccharide Purification. The details of our services are as follows:

• Selection of resin with appropriate functional groups or anionic/cationic resins according to the properties of the sample or charge characteristics.
• Samples are extracted, cleaned, and concentrated to obtain a solution suitable for entry into the purification system.
• Load the sample into the injector. Proper interaction between the polysaccharide and the IE resin is achieved by adjusting the specific parameters of the eluent. These conditions will be optimized in advance to improve the adsorption of polysaccharides.
• The polysaccharide interacts with the IE resin and is adsorbed, then the non-target molecules will be eluted. The product solution is collected and subsequently followed by procedures involving purification, concentration, and lyophilization.

Fig.2 Process of IECC-based polysaccharide purification. (CD BioGlyco)

Applications

• The purified polysaccharides provide an ideal material base for biocompatibility and bioactivity studies and assure the accuracy and reliability of these studies.
• Polysaccharides show significant potential for application in drug-controlled release technology as materials for the preparation of delayed-release drugs.
• In the field of medical devices, polysaccharides are widely used in the preparation of various types of medical devices, including biodegradable scaffolds, tissue-engineered scaffolds, artificial blood vessels, and repair materials.

Advantages of Us

• IEC is broadly applicable to all types of polysaccharides, including cationic and anionic polysaccharides.
• Based on the characteristics of the target polysaccharide and the complexity of the sample, we customize the selection of the appropriate column material, particle size, capacity, and gradient elution scheme for you to achieve the best purification results.
• We are committed to providing our clients with perfect after-sales support and service. Whether you encounter problems during your research or need further technical advice, our team of professionals is committed to providing prompt and helpful assistance to all inquiries.

With extensive research experience in the field of glycobiology, CD BioGlyco provides clients with comprehensive IECC-based polysaccharide purification services. We specialize in utilizing advanced technologies to help clients tackle challenges in research, bringing better scalability and lower production costs to clients. If you are interested in our services, please feel free to contact us for more details.

• Gel Column Chromatography-based Polysaccharide Purification Service
• Affinity Chromatography-based Polysaccharide Purification Service