The Structures of Xylooligosaccharides

Xylooligosaccharide belongs to a kind of oligosaccharide that consists of xylose units. They are present in bamboo shoots, fruits, vegetables, milk, and honey. Xylooligosaccharides are oligosaccharides composed of xylose residues connected by β-(1→4) linkages. They vary in the number of xylose residues involved in their formation, ranging from 2 to 10. These variations are known as xylobiose, xylotriose, and so on. Apart from xylose residues, xylooligosaccharide is commonly linked with other side groups such as α-D-glucopyranosyl uronic acid or its 4-O-methyl derivative, acetyl groups, or arabinofuranosyl residues. Branched xylooligosaccharides exhibit a wide range of biological properties due to the presence of these side groups. Xylooligosaccharides display the ability to maintain and promote normal microbiota balance. Therefore, they have shown excellent potential as candidate prebiotic compounds. Some studies have indicated that certain species of Bifidobacteria, particularly B. adolescents, are more effectively grown on xylooligosaccharides than fructooligosaccharides.

Fig.1 Structures of xylobiose. (Aachary & Prapulla, 2011)

Xylooligosaccharide Manufacture Service at CD BioGlyco

CD BioGlyco provides the Chemical Method and Enzymatic Method to produce xylooligosaccharides.

1. The chemical methods for the manufacture of xylooligosaccharides

We produce xylooligosaccharides by autohydrolysis from a variety of feedstocks, including hardwoods, softwoods, corncobs, barley hulls and barley, almond shells, corn fiber, and rice hulls. We treat xylan-containing lignocellulosic materials under suitable operational conditions to produce xylooligosaccharides. The hemicellulosic chains undergo a gradual breakdown process. This breakdown is facilitated by hydronium ions, which are produced through water autoionization and the generation of organic acids in the system. The soluble products, primarily oligosaccharides, are formed by this process. Meanwhile, the cellulose and lignin components remain in the solid phase with minimal chemical changes. Then we purify xylooligosaccharides by various approaches.

2. The enzymatic methods for the manufacture of xylooligosaccharides

We produce xylooligosaccharides by xylanase which hydrolyzes β-1,4 glycosidic linkages in xylan. Xylanase is made up of 1,4-β-d-xylan xylohydrolases, 1,4-β-xyloside xylanohydrolases, and debranching enzymes.

We achieve the manufacture of xylooligosaccharides from tobacco stalk xylan with xylanase preparations from Trichoderma longibrachiatum. We use a high-performance liquid chromatography (HPLC) system with a refractive index detector and a column oven to analyze the sugars we produce.

This process results in various undesirable components including soluble lignin, a large number of monosaccharides, and their dehydration product. Therefore, to produce high-purity xylooligosaccharides, we conduct extensive purification by solvent extraction, precipitation, adsorption, chromatographic separation, and membrane technology.

Subsequently, we analyze and characterize the products by various technologies.

Fig.2 Characterization methods of xylooligosaccharides. (CD BioGlyco)

Advantages of Us

• We possess a variety of technologies to analyze xylooligosaccharides to ensure their quality.
• We offer a diverse range of production methods for you to choose from.
• Our production processes are mature and highly efficient.

CD BioGlyco is a distinguished leader in the field of glycoscience. We specialize in the production of xylooligosaccharides. We are renowned for our expertise in providing Oligosaccharide Manufacture Service. Our outstanding research team ensures the highest quality output. If our services catch your attention, please don't hesitate to contact us.

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