Significance of Starch synthase (SS) Gene Editing

SS plays a crucial role in starch synthesis, catalyzing the formation of α-1,4 glycosidic bonds. It can transfer glucose residues from ADP-glucose to glucan nonreducing ends, and function to extend starch chains. Five types of SS isoforms have been identified from several plants.

Fig.1 The role of SS in starch synthesis. (Wang, et al., 2014)

Starch has a wide range of applications and can be used in papermaking, cosmetics, pharmaceuticals, etc. Since the limited properties of natural starch, the original starch can be changed by some chemical, biological means, etc. SS affects the composition, content, etc. of starch, so by mutating SS genes, we analyze the effects of SS mutants on starch as a way of modifying starch. Therefore, there have been some reports on SS gene modification, SS mutation causes changes in starch, structure, etc.

The Service is Endless

CD BioGlyco is experienced in genetic modification, led by the most professional team. We improve the structure, content, etc., of starch by constructing SS gene mutants that affect the process of starch synthesis. We will develop an experimental program according to the customer's requirements and can complete the whole experimental process or any part of it.

The general scheme of the experiment is to construct the corresponding vector after sequence comparison. Then we will infect the healing tissue of the recipient plant with Agrobacterium containing the corresponding vector to construct different SS mutant plants. After a fixed time of cultivation, the expression and enzyme activities of the relevant enzyme genes of the transgenic plants will be detected. The expression level of SS genes in transgenic plants will be studied in the post-transcriptional period, meanwhile. We also compare the differences between wild-type and experimental plants. So, the effect of this SS mutant on the starch and the synthesis process will be analyzed and concluded in the final analysis.

Fig.2 Experimental techniques. (CD BioGlyco)

Applications

• There are still a number of species in which SS genes have not been identified. Their functions have also not been verified. Gene editing technology helps researchers to validate the function of SS genes.
• The use of various gene editing techniques enables targeted regulation of starch properties, improvement of starch quality, or innovation of novel starches.
• Targeted regulation of SS genes using gene editing technology helps to improve grain yield and quality.

Advantages

• We have multiple gene editing capabilities. Multiple target gene loci can be edited at the same time.
• The gene editing technologies we employ are broad-spectrum, with no genetic, cellular, or species limitations. Gene editing technology can be used for multi-species gene editing.
• We have a well-established project management process and strict quality control system to ensure that projects are completed on time and efficiently.

CD BioGlyco provides SS gene modification customized service one-on-one according to the needs of each customer's research background and provides detailed experimental analysis reports. Starch synthesis is mainly regulated by four enzymes, in addition to SS, we also provide Starch-Branching Enzyme, ADP-Glucose Pyrophosphorylase gene modification services, etc. If you would like a detailed experimental consultation, please feel free to contact us.

• ADP-Glucose Pyrophosphorylase (AGPase) Modification
• Starch-Branching Enzyme Modification
• Debranching Enzyme Modification