The Structural Basis of Isoamylase (ISO)

Isoamylase (EC 3.2.1.68), belonging to the glycoside hydrolase subfamily GH13-11. It catalyzes the hydrolysis of the α-1,6-D-glucosidic linkages in amylopectin, glycogen, and derived oligosaccharides. ISO has high hydrolytic activity against macromolecular substrates such as pullulan and glycogen, while low hydrolytic activity for small molecule branched dextrin. Isoamylase is mainly composed of three domains: terminal domain, domain, and C-terminal domain. Domain A consists of helixes and an (α/β)₈-fold barrel structure. The C-terminal domain of isoamylase is composed of a small helix and a reverse fold, which is folded by 6 folded pieces to form a sandwich-type spatial structure. The two catalytic sites of glutamicacid/asparticacid (Glu/Asp) exist in the active center, which is an acid-base catalytic mechanism. ISO is used in food, feed, detergents, and other industries due to its high debranching efficiency and transglycosylation activity.

Fig.1 Overall structure of Isoamylase. (Woo E-J, et al., 2008)

ISO Treatment Services at CD BioGlyco

In the starch granule state, the enzyme modification maintaining the semi-crystalline structure is a challenge. At CD BioGlyco, we offer high-stability isoamylase-treated starch manufacture and detection services. We have always been well received by customers with quality service and reliable products. The services we offer are as follows:

• During the production process, we focus on maintaining the natural qualities. On the one hand, to ensure processability; on the other hand, to achieve the unique and distinctive gelatinization of the starch and the formation of a mechanically stable gel structure.
• At CD BioGlyco, our starch products are thoroughly studied in terms of morphology-scanning electron microscopy (SEM), structure-X-ray diffraction (XRD), heat-differential scanning calorimetry (DSC), process functions (solubility, hot paste viscosity, gel strength), and molecular properties-size exclusion chromatography-multi-angle light scattering-differential refractive index (SEC-MALS-DRI) to ensure the quality of production process.
• We customize modified starch according to your special needs, design the best production route, maximize yield, and characterize the product. You will get the analytical data reports analyzed by professionals.

Fig.2 Flow chart of isoamylase modification. (CD BioGlyco)

Applications

• Isoamylase-treated starch is used to prepare high-purity straight-chain starch.
• Isoamylase-treated starch starch is used to produce food additives.
• Isoamylase-treated starch is used to produce maltose and glucose syrup.
• Isoamylase-treated starch products are used as gelling agents.

Advantages

• Isoamylase-treated starch has acid and high-temperature resistance.
• Modified starch slows down aging, and prevents regeneration.

CD BioGlyco has a Carbohydrate Manufacturing platform to provide you with a high-performance and cost-effective process. We provide high-quality Modified Starch Manufacturing services to customers all over the world. We will continue to improve the quality of our services to better meet customer needs. If you are interested in our services, please feel free to contact us for more details.

• Amylo-1,6-Glucosidase Treatment
• Pullulanase Treatment