Stachyose Manufacture Service
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The Significance of Stachyose
Stachyose is one of the abundant carbohydrates in plants and belongs to the members of the raffinose family of oligosaccharides. It was first found in the rhizomes of Stachys tuberifera and coexists with other related oligosaccharides in miscellaneous organs of a large number of plant species. It is a tetrasaccharide that consists of sucrose with an alpha-D-galactosyl-(1->6)-alpha-D-galactosyl group attached to the 6-position of glucose. On a weight basis, stachyose is approximately 28% less sweet than sucrose. It is primarily applied as a bulk sweetener or for its functional oligosaccharide properties. In both mice and humans, stachyose has been proven to have a selective impact on the promotion of beneficial intestinal bacteria growth and the inhibition of pathogenic bacteria. Indeed, the production of stachyose holds great significance due to its beneficial properties.
Fig.1 The structure of stachyose. (CD BioGlyco)
Stachyose Manufacture Service at CD BioGlyco
CD BioGlyco provides a Biocatalytic Approach to manufacturing stachyose by constructing in vitro enzymatic systems. We conduct the cascade reactions by utilizing the cell lysate supernatant without the addition of the expensive UDP. Stachyose is produced through the sequential transfer of galactosyl units from galactinol to sucrose, a reaction catalyzed by the enzyme stachyose synthase. Our proposed system aims to reduce production costs by recycling and regenerating UDP and inositol to facilitate the conversion of sucrose into the desired product.
To avoid the need for enzyme purification and the supplementation of additional UDP, we employ cell extracts containing the expressed enzymes and a minimal amount of UDP in the reaction medium. This approach allows for effective production and ensures maintaining the catalytic activity of the enzymes. We utilize a stepwise reaction strategy to address mismatches in enzyme characteristics. In a word, we have successfully showcased an efficient method for biosynthesizing stachyose using an in vitro multienzyme system and a stepwise cascade reaction process.
Fig.2 The workflow of stachyose manufacture. (CD BioGlyco)
Applications
Due to its unique physicochemical properties and physiological effects, kestose has a wide range of potential applications in the food, pharmaceutical, cosmetics, feed, and brewing industries.
As a non-digestible oligosaccharide, kestose has important effects on the digestive physiology and nutrient metabolism of animals. Therefore, it can be used in the feed industry to regulate the gut flora, increase the number of beneficial bacteria, reduce harmful bacteria, improve piglet immunity, and enhance the absorption of mineral elements.
Kestose is safe and reliable, with no toxic side effects. Its clear structure and significant functional effects make it suitable for use in a variety of functional foods, such as bread, steamed buns, cakes, desserts, candies, dairy products, beverages, and ice cream, catering to different consumer needs.
Kestose has medicinal value in disease prevention, anti-aging, and other medical areas. It has potential preventive and therapeutic effects on various chronic diseases. Therefore kestose can be used as a targeted drug delivery. Moreover, kestose has high viscosity and strong water absorption. Hence it is useful to be a pharmaceutical excipient for dosage forms such as granules, capsules, tablets, and oral liquids.
Kestose can be used in skincare and cosmetic products, particularly in maintaining skin elasticity and plasticity and delaying skin aging. When combined with other anti-free radical substances, it even has better moisturizing and anti-aging effects. As a novel functional cosmetic ingredient, kestose has been applied in products such as toners and face masks.
Advantages of Us
- Our "three stages in one pot" strategy is novel.
- Our biocatalytic method provides new opportunities for the production of valuable galactosyl oligosaccharides that are difficult to obtain through traditional plant extraction methods.
- Our method is highly efficient for the biosynthesis of stachyose utilizing an in vitro multienzyme system.
CD BioGlyco is a pioneering biotechnology company with expertise in stachyose manufacturing. With our unparalleled experience, we offer a comprehensive One-Stop Solution for Carbohydrate Manufacture. Our company is led by a renowned research team in the field of glycoscience. We excel at optimizing every aspect of the process, utilizing state-of-the-art technology and meticulous attention to detail. If our services have intrigued you, we encourage you to contact us promptly. We eagerly want to have the chance to discuss how we can fulfill your needs.
Reference
- Liu, Y.; et al. Regulatory effects of stachyose on colonic and hepatic inflammation, gut microbiota dysbiosis, and peripheral CD4+ T cell distribution abnormality in high-fat diet-fed mice. Journal of Agricultural and Food Chemistry. 2019, 67(42): 11665-11674.
For research use only. Not
intended for any clinical use.
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