Principle of Enzyme Membrane Reactors

Enzyme membrane reactor technology combines enzyme and membrane techniques for efficient oligosaccharide production. In enzyme-membrane reactors, enzymes act as catalysts, transforming polymers into valuable oligomers, which are subsequently purified by membrane separation. The membrane functions as a selective barrier, permitting molecules of specific sizes to pass through while blocking others. This enables the production of oligosaccharides with precise molecular weight distributions. The enzyme membrane reactor demonstrates several advantages, including high efficiency, minimal side effects, and serves as an excellent method for preparing bioactive oligosaccharides.

Fig 1 Methods for obtaining primary and secondary oligosaccharides. (Su, et al., 2020)

Enzymatic Process for Oligosaccharide Manufacture at CD BioGlyco

As a company in the field of glycoscience, CD BioGlyco provides a variety of advanced Technologies for Oligosaccharide Manufacture. Among them, the enzymatic process is currently the most widely used technology for the Manufacture of Oligosaccharides, including enzymatic degradation/hydrolysis for oligosaccharide manufacture, enzymatic synthesis for oligosaccharide manufacture, enzymatic transglycosylation for oligosaccharide manufacture, enzymatic isomerization for oligosaccharide manufacture, and enzymatic epimerization for oligosaccharide manufacture.

Enzymatic Degradation/Hydrolysis for Oligosaccharide Manufacture

Enzymatic degradation/hydrolysis is an important pretreatment step in the enzymatic production of oligosaccharides. The steps of enzymatic degradation/hydrolysis typically involve pretreatment followed by microbial or chemical conversion. Importantly, the enzymatic degradation/hydrolysis approach also allows direct production of oligosaccharides other than sugar monomers. Experts at CD BioGlyco work tirelessly to provide enzymatic degradation/hydrolysis processes for oligosaccharide manufacture that meet clients' needs.

Enzymatic Synthesis for Oligosaccharide Manufacture

Using glycosidase as a catalyst and disaccharide as a substrate to enzymatically synthesize oligosaccharides is a feasible technology. Notably, enzymatic cascade reactions are powerful techniques for developing environmentally friendly, cost-effective oligosaccharide synthesis processes. CD BioGlyco provides comprehensive and advanced enzymatic synthesis, especially enzymatic cascade synthesis technology for oligosaccharide production.

Enzymatic Transglycosylation for Oligosaccharide Manufacture

CD BioGlyco provides enzymatic transglycosylation of oligosaccharides produced with glycosidases. Glycosidases catalyze dehydrolysis or transglycosylation by a retention mechanism. When disaccharides are used as substrates, transglycosylated products are formed by self-condensation.

Enzymatic Isomerization for Oligosaccharide Manufacture

Enzymatic isomerization is an advanced technology for producing lactulose. In this process the cellobiose 2-epimerase isomerizes the glucose moiety of cellobiose or glucose, converting lactose to lactulose. This thermostable enzyme allows the reaction to be performed at a high temperature, which increases the solubility of the substrate and the reaction rate of the enzyme. CD BioGlyco provides high-quality enzymatic isomerization for oligosaccharide manufacture to maximize the production of lactulose.

Enzymatic Epimerization for Oligosaccharide Manufacture

Enzymatic epimerization is an environmentally friendly technology for producing oligosaccharides. The technology selects cellobiose 2-epimerase as the catalyst and disaccharide as the substrate to produce the rare oligosaccharide epilactose. CD BioGlyco provides enzymatic epimerization service to develop an environmentally friendly process to manufacture oligosaccharides.

Applications

• Functional food and prebiotics: Enzymatic processes for oligosaccharide manufacture are widely used to produce prebiotic compounds that support gut health.
• Pharmaceuticals and biopharmaceuticals: Enzymatically synthesized oligosaccharides are conjugated to therapeutic proteins or peptides to enhance their stability, pharmacokinetics, and targeting properties.
• Diagnostics and biomarker discovery: Enzymatic processes enable the creation of custom-designed oligosaccharide structures for diagnostics and biomarker discovery.
• Biocatalysis and green chemistry: Enzymatic processes for oligosaccharide manufacture align with the principles of green chemistry.

Advantages

• The process of producing oligosaccharides by the enzymatic process is environmentally friendly.
• Enzyme membrane reactor technology has the advantages of high efficiency and fewer side effects.
• Enzymatic membrane reactors help enzymatic processes increase productivity and oligosaccharide yield.

CD BioGlyco provides an integrated One-Stop Solution for Carbohydrate Manufacture. Our advanced physical-/chemical process for oligosaccharide manufacture and high-quality service will fully meet your needs. If you'd like to know more, please feel free to contact us.

• Physical-/Chemical Process for Oligosaccharide Manufacture
• Chemoenzymatic Process for Oligosaccharide Manufacture
• Separation Process for High-Purity Oligosaccharide Manufacture
• Enzymatic Degradation/Hydrolysis for Oligosaccharide Manufacture
• Enzymatic Synthesis for Oligosaccharide Manufacture
• Enzymatic Transglycosylation for Oligosaccharide Manufacture
• Enzymatic Isomerization for Oligosaccharide Manufacture
• Enzymatic Epimerization for Oligosaccharide Manufacture