Applications of Affinity Chromatography (AC)

AC is a technical method to separate and purify the biomolecules. AC is extensively utilized to study the binding between biomolecules. Immobilizing a ligand to study the receptor to which it binds, helps to understand the signaling pathways of biomolecules as well as the mechanism of disease occurrence. AC is also used in high throughput screening (HTS) platforms. During drug discovery, AC can be used to screen for binding between drug candidates and target proteins, which helps to quickly screen compounds with potential therapeutic effects. Moreover, AC is also utilized for sugar purification, whereby specific types of sugars can be selectively captured and purified for structural characterization and functional studies of sugars. Overall, the current state of research in AC in the biological sciences is highly active. The application of AC in research will continue to expand with further development and improvement of the technology.

Fig.1 Principle of AC. (Coskun, 2016)

AC-based Polysaccharide Purification Service at CD BioGlyco

Polysaccharides have various significant functions in biology, including energy storage and supply, structural support, as well as lubrication and protection. These functions are critical to the normal physiological activities of an organism. CD BioGlyco focuses on Polysaccharide Manufacturing services including Starch Manufacturing and Glucan Manufacturing. We hope to provide efficient AC-based polysaccharide purification services for your glycan research, and the details of our service process are as follows:

• Lectin AC-based purification services

Lectins are a class of proteins that specifically bind sugar moieties. At CD BioGlyco, we pass samples through a lectin affinity resin, the polysaccharide binds to the lectin, and other impurities are eluted. The target polysaccharide is then dissociated from the affinity resin by adjusting the elution conditions.

• Ion exchange (IE) AC-based purification services

Polysaccharides interact with charged ion exchange matrices, and we utilize this to perform purification. Positive IE matrices are commonly used to separate anionic polysaccharides, while negative IE matrices are commonly used for the purification of cationic polysaccharides. We control the binding and dissociation of polysaccharides with the IE matrix by adjusting the ion concentration, pH, and other conditions of the elution buffer to achieve the separation and purification of polysaccharides.

Fig.2 Process of AC-based polysaccharide purification. (CD BioGlyco)

Applications

• Covalent modification or physical adsorption of polysaccharides can be used to make them interact with specific biomolecules, thus enabling the detection and analysis of target substances.
• Polysaccharides can be used as coating materials for textiles, adding waterproof, antibacterial, and flame-retardant features to textiles.
• Polysaccharides have the potential to improve soil structure and increase water retention capacity. In agriculture and horticulture, polysaccharides are widely used in soil improvement and measures to increase soil water retention capacity.

Advantages of Us

• The unique selectivity of AC enables efficient purification of target polysaccharides from complex mixtures without interference from other cellular components or impurities.
• Our researchers have a wide range of professional knowledge and experience with deep expertise in the field of polysaccharide purification. They develop the best purification protocols and optimize the experimental processes and parameters according to the characteristics and needs of the target polysaccharides.

CD BioGlyco offers comprehensive AC-based polysaccharide purification services to help clients delve deeper into the properties and functions of polysaccharides. Our professional research team provides clients with various types of analytical data depending on their specific needs. If you are interested in our services, please feel free to contact us for more information.

• Ion Exchange Column Chromatography-based Polysaccharide Purification Service
• Gel Column Chromatography-based Polysaccharide Purification Service