Principle of Salt Fractionation (SF)

SF is a frequently used purification approach, it exploits the property of polysaccharides to form complexes with salts to achieve purification. This separates the polysaccharide from other impurities in the solution. For instance, by adding an appropriate amount of sodium acetate (NaOAc) to the mixture, the polysaccharides can be induced to form complexes with sodium acetate, which induces precipitation and separation of the polysaccharides. By centrifugation and other separation methods, the polysaccharide precipitate can be isolated from other components in the solution. SF, as a simple, economical, and highly useful purification method, is particularly suitable for high molecular weight polysaccharides, such as polysaccharide-protein complexes. It not only removes impurities in the solution but also enriches the target polysaccharide at the same time, improving the yield and purity.

SF-based Polysaccharide Purification Service at CD BioGlyco

CD BioGlyco has a one-stop SF-based polysaccharide purification service. The process of our services is as follows:

• Raw Material Preparation
  First, a solution containing the target polysaccharide is prepared. The solution may be a polysaccharide solution obtained by extraction from a biological source or a polysaccharide-rich solution obtained by pre-treatment and extraction.
• Purification Process
  Gradually add a suitable salt to the solution. We will determine the type and concentration of salt based on polysaccharide properties and interactions. The solution is stirred to promote the interaction between the polysaccharide and the salt, and the conditions of the solution are adjusted, e.g., adjusting the solution pH, temperature, etc., to induce the formation of complexes between the polysaccharide and the salt.
• Sedimentation and Separation
  After complex formation, the complex is precipitated from the solution using a centrifuge. The speed and time of centrifugation are adjusted according to the properties of the polysaccharide and salt to ensure adequate precipitation of the complex. The precipitate obtained by centrifugation is separated from the supernatant, and a product of higher purity could be obtained by repeating the above steps.

Fig.1 Flow chart of SF-based polysaccharide purification. (CD BioGlyco)

Applications

• Polysaccharides exhibit the ability to modulate the immune system and anti-inflammatory responses and therefore can be used as immunomodulators or immune-enhancers in the study of immune disorders, tumors, and infectious diseases.
• Polysaccharides are frequently employed in cosmetics to provide moisturizing, antioxidant, and anti-aging benefits.
• Polysaccharide purification technology is used for polysaccharide components in plant extracts for the preparation of cosmetic products with moisturizing, anti-aging, and restorative functions.

Advantages of Us

• The SF operates without introducing other impurities, thus ensuring the high purity of the products.
• SF does not require a large number of consumables, making it relatively inexpensive and cost-effective.
• Our state-of-the-art laboratory equipment and specialized technical team ensure accurate, safe, and efficient handling of samples.

CD BioGlyco provides high-quality Polysaccharide Manufacturing Services to our clients. Our SF-based polysaccharide purification service provides professional results reporting to clients. As each project has different requirements, please contact us and our experts will discuss the details with you.

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