What Is DB

Degree of branching refers to the number of A chains in each B chain, i.e. A: B value. There are three kinds of chains in the branched chain, A, B, and C. The A chain is bound to other chains by a glycosidic bond with a reducing end, but not bound by other chains; the B chain is bound to other chains and at the same time is bound by other chains; the C chain is bound to other chains with a reducing end. There is only one C chain in each branched chain, the other end of the C chain is a non-reducing group. A and B chains only have non-reducing groups.DB is one of the most basic structural parameters of the sugar chain. Branching-based analysis schemes may provide a viable way to resolve long and complex carbohydrate chains.

Fig.1 Schematic representation of the branch connections. (Zhong et al., 2021)

DB Analysis Services at CD BioGlyco

Branching analysis of β-glycan is a key part of understanding the composition and conformational relationships of sugar chains. At CD BioGlyco, we have a variety of methods to assist and decipher highly branched polysaccharides in research. We provide the following services to help our customers quickly and efficiently determine the percentage of branching.

• We offer an enzymatic combined capillary electrophoresis assay service. First, fluorescently label debranched branched starch, and then determine the reducing power using the capillary electrophoresis instrument's laser-induced fluorescence detector (LIF). This method reduces the number of reaction steps in the original enzymatic assay and improves the accuracy of the results.
• MS-assisted determination has been a powerful tool for structure analysis in recent years. We isolate the product by chemical degradation to produce oligo- or monosaccharides or even smaller fragments. Then, perform mass spectrometry/flame ionization detection (MS/FID) after multi-step derivatization of the product, which is a commonly used method for structure analysis of polysaccharides.
• Furthermore, we also provide nuclear magnetic resonance (NMR) spectroscopy to analyze the structure of various dextrans, which quickly and accurately determine their linkages.

Fig.2 Schematic diagram of DB analysis. (CD BioGlyco)

Applications

• DB analysis of beta-glucan structures is the basis for customized structural analysis.
• DB analysis of beta-glucan structure can be applied to Isomer judgment.

CD BioGlyco provides efficient and accurate Beta Glucan Structural Analysis Service, which is mainly divided into the analysis of DB analysis, DP/MW Analysis By SEC, and Linkage Ratio Analysis. Combined with advanced methodological strategies, efficient and accurate service has been recognized by customers in many countries. If you would like to learn more about the Beta-Glucan Manufacture or Quantification services, please do not hesitate to contact us.

• DP/MW Analysis By SEC
• Linkage Ratio Analysis