The Specificity of Transferase

Transferase is divided into Amylomaltase and Cyclodextrin Glycosyltransferase. Amylomaltase of the GH77 family belongs to the large superfamily of α-Amylases, enzymes characterized by a central (β/α)₈ catalytic domain (usually referred to as domain A). Cyclodextrin glycosyltransferase belongs to the new subfamily, with multiple substrate specificity. It hydrolysate many different dextrins, starch, pullulan, and other substrate at the same time, and have a high hydrolysis rate. Transferase plays an essential role in starch treatment, ringing oligosaccharide products into dextrins of different sizes, and further degrading them into maltose and standard products. It is applied in many ways to prepare starch, products, by modifying starch to obtain different products. Modified starch is an important ingredient widely used for stabilization, gelling, texturizing, thickening, and water retention of foods.

Fig.1 Physiological role of transferase. (Park, et al.,2000)

Transferase Treatment Service at CD BioGlyco

With the development of modified starch manufacture, more and more enzymes are applied to modify starch to obtain different product fragments according to substrate specificity. CD BioGlyco uses a single swing compound enzyme method to modify the starch, through the optimization of the process conditions, and the detection of starch performance, a series of different products were obtained.

At CD BioGlyco, we have several modified methods to provide clients with customized modified starch. For example, we provide the production of transferase-treated starch at sub-gelatinization temperatures and different pH conditions. Moreover, we offer analysis of biochemical properties, thermal and structural analysis, the content of oligosaccharides, domain structure, and molecular features for modification of starch. We treat the starch structure according to customer needs and prepare porous starch or non-porous starch. We are capable of providing efficient and reliable services to accelerate our clients' starch research by shortening project time.

Fig.2 Transferase treatment services. (CD BioGlyco, 2023)

Applications 

• Transferase-treated starch can be used to produce highly branched starch.
• Adding transferase-treated starch in frozen foods improves product stability.
• Transferase-treated starch can be used in bread production to reduce hardness.

Advantages

• Transferase-treated starch products have high heat resistance and stability.
• Transferase-treated starch products have no bacterial contamination in the modification process.
• Transferase-treated starch inhibits regeneration in the modification process.
• Transferase-treated starch improves the quality of starch-based foods in the food industry.

CD BioGlyco has a professional scientific research team and first-class experimental equipment in the field of Carbohydrate manufacturing. Our professional team tailors the plan according to the needs of customers and provides the most thoughtful and comprehensive Starch Modification Manufacturing Services. Specifically, includes Physical Modification, Chemical Modification, and Enzymatic Modification. If you are interested in our services, please contact us for more details.

• Endoamylse Treatment
• Exoamylase Treatment
• Debranching Enzyme Treatment
• Amylomaltase Treatment
• Cyclodextrin Glycosyltransferase Treatment