The Process of Alkaline Protease Production

Alkaline proteases are classified as serine-containing or metallo-type enzymes and are extensively studied for their broad applications across industries. Recently, these proteases have found utility in enhancing starch yield and quality from raw grain materials. Bacteria and fungi are the primary sources of alkaline proteases, and their extracellular nature allows for easy separation from microbial cells through centrifugation or filtration, resulting in crude enzyme filtrate supernatants. Among microbial sources, Bacillus sp. is commonly utilized for producing industrially significant alkaline proteases. Their widespread use and ability to improve starch manufacturing make them valuable tools in the industry.

Fig.1 The production process of alkaline protease enzyme from microorganisms. (CD BioGlyco)

Alkaline-Protease Treatment for Starch Manufacture Service at CD BioGlyco

Proteases, also known as peptidyl-peptide hydrolases, are important industrial enzymes found in a variety of industries. CD BioGlyco creates wet-milling starch production services that include alkaline protease treatment. The previous study has demonstrated that alkaline protease can improve rice starch separation from protein. The remaining protein content of rice flour protein can be successfully decreased by hydrolysis, cellulase, and defatting methods. Besides rice, proteases are also used in Other Native Starch Manufacturing. Moreover, clients will find Wet-Milling Starch Manufacturing and Dry-Milling Starch Manufacturing services on our websites. Alkali Wet-Milling and Conventional Wet-Milling also are two commonly used technologies for predicting native starch.

Fig.2 Two essential types of alkaline proteases. (PDB)

Applications of Alkaline-Protease Treatment

• Breakdown of proteinaceous impurities
  Alkaline proteases are used to hydrolyze and break down proteinaceous impurities present in raw materials, especially in cereal-based starch sources. These proteases target proteins, effectively reducing their levels and improving the purity of the starch extract.
• Enhancing starch yield
  Alkaline proteases aid in disrupting the protein-starch complexes in the raw materials, resulting in increased starch yield during extraction. This process enables better release of starch granules, thereby maximizing the starch recovery.
• Improving starch purity and quality
  By selectively degrading proteins, alkaline proteases contribute to improving the purity and quality of the extracted starch. This purification step helps eliminate unwanted components and enhances the functional properties of the starch, making it more suitable for various applications.

Advantages of Us

• We provide custom alkaline-protease treatment services for native starch for our clients according to their requirements.
• Besides alkaline-protease treatment, we also provide the dry-milling process for starch manufacturing.
• Our R&D team is highly responsive and proactive, anticipating clients' needs and going above and beyond to ensure a positive experience and satisfaction.
• Our lab team exhibits strong attention to detail and follows rigorous quality control measures, ensuring the accuracy and precision of our experimental procedures and analysis.

CD BioGlyco provides excellent and custom alkaline-protease treatment wet-milling processes for starch manufacturing based on our high-level lab. If you have any questions or require details information, please feel free to contact us. Our team appreciates inquiries worldwide and we will try our best to help clients with professional knowledge.

• Conventional Wet-Milling for Starch Manufacture
• Alkali Wet-Milling for Starch Manufacture