What Is Depolymerization?

Depolymerization of polysaccharides is a process in which intramolecular glycosidic bonds break and the polymerization degree of polysaccharides decreases continuously, and then oligosaccharides or monosaccharides are produced. It is a simple method to produce monosaccharides based on polysaccharides.

Different Depolymerizing Methods

Many depolymerization methods have been proposed, including chemical depolymerization, physically-assisted hydrolysis, and biodegradation, among which chemical and hydrolytic enzymatic depolymerizations are commonly used to obtain monosaccharides. Many physically-assisted hydrolysis methods have been reported such as microwave, ultrasound, irradiation, dynamic high-pressure microfluidisation, and induced electric field degradation, but most of these methods can not directly and completely depolymerize polysaccharides into monosaccharides.

Fig.1 Reactional mechanism of radical and acid polysaccharide depolymerization. (Barreteau, et al., 2006)

Hydrolytic Enzymatic/Chemical Depolymerization Service

CD BioGlyco continues to innovate and improve monosaccharide manufacture technologies, closely following the international frontier research. We are committed to applying the most scientific technology in monosaccharide production and intend to provide clients with the most efficient, economical, and optimized production process and the best quality monosaccharides. We have different polysaccharide depolymerization strategies:

• Hydrolytic Enzymatic Depolymerization Service

Enzymatic depolymerization can selectively cut off the beta-(1,4)-glycosidic bond, and has the advantages of mild reaction conditions, no reaction reagents, and a fast degradation rate compared with the chemical methods. At present, more than 30 specific and non-specific enzymes have been found to catalyze polysaccharide hydrolysis, such as beta-glucosidase, alpha-amylase, cellulase, pectinase, and xylanase.

In the process of hydrolytic enzymatic depolymerization, the type and concentration of hydrolase, temperature, pH value, etc. are crucial variables. CD BioGlyco constantly explores and optimizes conditions in the production process, and strives to perfect each monosaccharide production.

• Chemical Depolymerization Service

The chemical depolymerization of polysaccharides includes acid hydrolysis, acid methanolysis, alkaline hydrolysis, ionic liquid hydrolysis, oxidation hydrolysis, acetolysis, etc. The first two are commonly used, and sulfuric acid, trifluoroacetic acid, and hydrochloric acid are widely used in the acid hydrolysis method. Acid methanolysis can gently hydrolyze glycosidic bonds within a one-step procedure, and the common reagents are hydrochloric and methanol.

Applications

• Obtain monosaccharides from polysaccharides or oligosaccharides
• Mass production of monosaccharides with a single structure and high-purity
• Food, pharmaceutical, and cosmetic industry development

Highlights

• Efficient and fast production process
• Mild production conditions
• Alternative methods to obtain monosaccharides

CD BioGlyco integrates a variety of carbohydrate molecules and related products that are essential for carbohydrate research. Except for monosaccharide manufacture service, we also provide Oligosaccharide Manufacture Service and Polysaccharide Manufacture Service. If you are interested in our services, please feel free to contact us.

• Isomerization via Immobilized Enzymes/Microorganisms
• Microbial Fermentation
• Chemical Production