The Substrate Specificity of Debranching Enzyme (DE) Treatment

The DE efficiently and exclusively cut the α-1,6-glycosidic bond, thus cutting out the entire collateral to form amylose. Due to the amylopectin content in the starch being relatively high, other amylases can not hydrolyze α -1,6-glycosidic bonds in amylopectin or hydrolysis efficiency is very low. So, the α-1,6-glycosidic bond in starch becomes a hindering factor in starch hydrolysis and needs the participation of DE. According to the difference in substrate specificity, DE is divided into amylo-1,6-glucosidase, isoamylase, and pullulanase. According to the similarities and differences in protein structure, it is a typical (β/α)₈-barrel structure, belonging to the GH 13 family. DE is widely used in the starch processing industry.

Fig.1 Protein structures and the cleavage method of debranching enzymes. (Xia, et al., 2021)

DE Treatment Services at CD BioGlyco

Because of the unique glycosidic bond-breaking properties of DEs, treatment of starch by DEs is warranted. CD BioGlyco has a Starch Manufacturing platform to provide you with a high-performance and cost-effective process. We provide high-quality and efficient DE-modified starch services, and we offer also DE-treated starch products with high catalytic activity and high specificity.

According to the difference in substrate specificity, we provide DE treatment services including Amylo-1,6-glucosidase, Isoamylase, and Pullulanase, resulting in changes in particle morphology, molecular structure, crystal type, crystallinity, solubility, gelatinization properties, and rheological properties of modified starch. Each step of the modification process is strictly controlled. In addition, our company offers detailed analytical services, such as differential scanning calorimetry, X-ray diffraction (XRD) analysis, dynamic light scattering detector, etc.

Our company offers efficient and fast production lines to meet the batch requirements of our customers. We focus on natural quality and provide you with different specifications of the packaging. Our global business and solid expertise make us a trusted partner for global glycobiology researchers.

Fig.2 The major classification of the debranching enzymes treatment. (CD BioGlyco)

Applications

• Deep processing of DE-treated starch produces more products such as amino acids, organic acids, and alcohol.
• DEs are added in the livestock and poultry breeding industry, to improve the body's digestive ability.

As a professional Carbohydrate Manufacturing company, CD BioGlyco provides high-purity Native Starch manufacturing and Modified Starch manufacturing services to customers all over the world. In addition, we provide efficient Endoamylse Treatment, Exoamylase Treatment, and Transferase Treatment services. We are capable of providing efficient and reliable services to accelerate our clients' scientific research by shortening project time. If you are interested in our service, please contact us further to introduce the specific service in detail.

• Endoamylse Treatment
• Exoamylase Treatment
• Transferase Treatment
• Amylo-1,6-Glucosidase Treatment
• Pullulanase Treatment
• Isoamylase Treatment