Diverse Effects of Isomaltooligosaccharide

Isomaltooligosaccharide belongs to the type of starch-derived sugar. It is primarily composed of four or more glucose molecules. These glucose molecules are linked together by α-1,2, α-1,3, α-1,4, and α-1,6 glucosidic bonds. These carbohydrates with different degrees of polymerization include panose, isopanose, isomaltotriose, nigerose, and so on. It is a naturally occurring component in fermented foods and is commonly found in the human diet. For example, fermented sourdough bread and kimchi contain isomaltooligosaccharide. The sweetness level of the sugar is about 50% compared to sucrose. Isomaltooligosaccharide is a multifunctional sugar that possesses diverse effects on digestive health. Isomaltooligosaccharide functions as a prebiotic, reduces flatulence, and possesses a low glycemic index. In addition, it has been observed to prevent dental caries in animals. Therefore, it is of great significance to produce isomaltooligosaccharide.

Fig.1 Chemical structures of isomaltooligosaccharide. (CD BioGlyco)

Isomaltooligosaccharide Manufacture Service at CD BioGlyco

CD BioGlyco provides two approaches to produce isomaltooligosaccharides by Enzymatic Processes.

• This strategy involves the process of simultaneous saccharification and transglucosylation of liquefied starch using fungal α-amylase and α-glucosidase. We adopt this approach for the production of lignocellulosic substrates.
• Our strategy coupling of saccharification and transglycosylation steps essentially reduces the process times, hence improving productivity and alleviating the inhibition of glucose. Furthermore, the simultaneous execution of both reactions within one pot simplifies the process handling.
• The glucose produced during the saccharifying enzyme process is prevented from accumulating. This is achieved by concurrent transglycosylation with α-glucosidase, resulting in the production of oligosaccharides. This process effectively reduces the potential inhibition caused by the liberated glucose.
• In the process, glucose produced by the saccharifying enzyme is immediately trans glucosylated by α-glucosidase to form oligosaccharides. This prevents the accumulation of glucose and reduces potential inhibition caused by liberated glucose.

Fig.2 The manufacture of isomaltooligosaccharide by simultaneous saccharification and transglycosylation. (CD BioGlyco)

• To produce isomaltooligosaccharides, we have introduced the aglA gene from A. niger into S. cerevisiae. The expression of this gene is regulated by a galactose-inducible promoter, allowing us to control the production of α-glucosidase in the yeast. Recombinant yeast cells expressing the aglA gene produce extracellular α-glucosidase activity. 
• About half of them appear as cell-bound whereas the other half are released into the culture medium. Using maltose as the substrate, the cells derived from cultures of recombinant yeast strains expressing aglA constructs are employed for the successive batch production of isomaltooligosaccharides. This engineered yeast we construct enables highly efficient production of isomaltooligosaccharides with excellent yields.

Advantages of Us

• We have diverse methods to produce isomaltooligosaccharides.
• Our production methods are simple.
• Our production process is efficient.
• We produce isomaltooligosaccharides with excellent yields.

Applications

• Isomaltooligosaccharide is widely used in the candy, pastry, beverage, and dairy product industries because of its ability to stimulate the growth of Bifidobacterium.
• Isomaltooligosaccharide can be used in the baking industry due to its ability to enhance the taste and texture of the product.
• Isomaltooligosaccharide can be used in the wine-making industry due to its ability to enhance wine's flavor, quality, and bio-functions.
• Isomaltooligosaccharide can be used in the feed industry due to its role as a substitute for living microorganisms.

CD BioGlyco is the unrivaled leader in isomaltooligosaccharide manufacture. Our company is proud to provide a comprehensive One-Stop Solution for Oligosaccharide Manufacturing. Our company is trusted by esteemed experts in the field of glycobiology. We utilize innovative approaches to achieve outstanding results. If our services resonate with your needs, please contact us for more information.

Reference

• Gourineni, V.; et al., Gastrointestinal tolerance and glycemic response of isomaltooligosaccharides in healthy adults. Nutrients. 2018, 10: 301.

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