Advantages of Microbial Fermentation to Synthesize Glycolipids (GLs)

GLs are lipids containing sugars but not phosphoric acid. GLs have unique properties, such as low toxicity and biodegradability. The most intensively researched are rhamnolipid (RL), trehalose (TL), and sophorolipid (SL). Currently commercially produced glycolipid biosurfactants are mainly synthesized by microbial fermentation. The products are usually mixtures of different branched alkyl chains or different alkyl chain lengths. There are more advantages to synthesizing glycolipids from scratch through microbial fermentation. The process allows for a range of different renewable substrates or by-streams and waste streams from other industries to be used as substrates without having to be part of the final product. Also, GLs synthesized using this method may contain rare or unusual fatty acids or sugars. By using sidestreams or waste streams, overall process economics can be improved and waste generation can be reduced.

Tab.1 Examples of various yeasts producing glycolipids. (Jezierska, et al., 2018)

Our Services

CD BioGlyco makes full use of its well-established experimental platform and the team's strong R&D capability to optimize the glycolipid synthesis process.

• GL production services

Although GL has a wide range of applications, there are some technical barriers to GL manufacturing, such as low productivity and highly heterogeneous products. To reduce production costs and maximize GL purity, continuous optimization of the GL production process is required. We provide GL production services.

We optimize the microbial fermentation process through the following main areas:

• Microbial modification: optimization of GL synthase, optimization of metabolic pathways, provision of precursor supply, genetic modification
• Fermentation media substrates: searching for renewable and inexpensive wastes as substrates
• Fermentation processes: medium composition, temperature, pH, dissolved oxygen, carbon source, nitrogen source, carbon/nitrogen ratio, metal ions, agitation speed, biomass
• Separation process: centrifugation, extraction, filtration, etc.

In order to expand the application scope of GLs, we use chemical or enzyme modification to improve their physicochemical properties, biological activity, etc.

Fig.1 Microbial fermentation process of glycolipids. (CD BioGlyco)

Bacterial Fermentation Process-based Glycolipid Manufacture Service

Bacteria often produce GLs of various structures in addition to macromolecular lipopolysaccharides. CD BioGlyco produces RL primarily through the bacterial fermentation process.

Yeast Fermentation Process-based Glycolipid Manufacture Service

Yeast is currently used in the production of GLs. CD BioGlyco optimizes the yeast fermentation process parameters and the isolation and purification methods, resulting in a highly pure SL.

Chemical Post-Treatment-based Glycolipid Manufacture Service

Incorporating a chemical modification step in the microbial fermentation process generates novel GLs. CD BioGlyco is based on extensive chemical and biological experience in modifying glycolipids by different chemical means to improve the biological activity of glycolipids.

Enzymatic Post-Treatment-based Glycolipid Manufacture Service

Enzymatic modification is a mild reaction with fewer by-products. It can selectively change the specific structure to synthesize new GL derivatives. CD BioGlyco has an extensive experience in enzymatic modification, and develops detailed GL modification programs according to customer requirements.

• RL and SL manufacture service

We offer RL Manufacture Service and SL Manufacture Service. High-purity, green, and better-performance GL products can be obtained through our optimized process.

Applications

• High-quality GL helps researchers to explore the application of GL in environmental ecologies such as the remediation of contaminated soil, wastewater treatment, and degradation of waste oil.
• In biomaterials preparation, efficient GL production techniques are used to study the preparation and application of specific biomaterials containing glycolipids.
• Efficient GL production technology is also important in pharmaceuticals. Synthesized high-quality GLs can be used to study their applications in inhibiting tumor cell proliferation, immunomodulation, etc.

Highlights

• We have a dedicated research team for the GL process to optimize the GL production process. All GL products are quality-tested and have very low CMC concentrations.
• Our researchers are enthusiastic, skillful, and attentive. We will quickly develop valuable and personalized experiments to meet the needs of our customers.
• We ensure that the overall process is environmentally friendly and that all raw materials for SL synthesis are of natural plant origin. There is no chemical reaction or chemical reagent added in the synthesis process.

CD BioGlyco uses optimized microbial fermentation to produce bio-green glycolipid products. We aim to serve customers in different fields, including universities, R&D organizations, cosmetic companies, pharmaceutical companies, and so on. In addition to the microbial fermentation process, we also provide Chemical Synthesis-based Glycolipid Manufacture Service and Enzymatic Synthesis-based Glycolipid Manufacture Service. Welcome to contact us for ordering GLs. We are ready to provide glycolipid synthesis services for you.

• Chemical Synthesis-based Glycolipid Manufacture Service
• Enzymatic Synthesis-based Glycolipid Manufacture Service
• Bacterial Fermentation Process-based Glycolipid Manufacture Service
• Yeast Fermentation Process-based Glycolipid Manufacture Service
• Chemical Post-Treatment-based Glycolipid Manufacture Service
• Enzymatic Post-Treatment-based Glycolipid Manufacture Service