Functions of Cyclodextrin Glycosyltransferase

Cyclodextrin glycosyltransferase (CDTase, EC 2.4.1.19) is an Endoenzyme, a member of the α-amylase family. This enzyme catalyzes four kinds of reactions by cleaving α-1,4 glycosidic bonds present in the inner part of a polysaccharide chain. CDTase usually has minor hydrolysis activity and mainly catalyzes three transglycosylation reactions: cyclization, coupling, and disproportionation. In addition to the hydrolysis of α-1,4 and 1,6 glycosidic bonds, new α-1,3,α-1,4, and α-1,6 bonds can also be formed. The ability of CDTase to produce cyclodextrins from starch by cyclization is the basis for its industrial application. This is the basis for its industrial application. CDTase-treated starch products are non-toxic, colorless, and odorless. It has a wide range of applications in the fields of food, medicine, agriculture, textile, environmental protection, cosmetics, biotechnology and analytical chemistry, etc.

Fig.1 The catalytic specificity of CDTase. (Aroob, et al., 2019)

CDTase Treatment Service at CD BioGlyco

CDTase modification of Native Starch is necessary due to its wide range of applications and market demand. The technologies we provide for enzymatic modification mainly include the optimization of preliminary process parameters, sample preparation, extraction, separation, and determination of related indicators. Based on production processes, optimized strategies include the optimal reaction temperature and pH, enzymatic biochemical features, thermal modifications, and structural modifications. Our expert technicians are capable of providing customized processes for customers all over the world.

We not only provide starch modification services but also include comprehensive product characterization services. For treated starch, we confirm the enzyme modification by microscopic analysis of starch granules, resulting in an irregular surface and a structure of small pinholes. We studied in terms of morphology-scanning electron microscopy (SEM), structure-X-ray diffraction (XRD), heat-differential scanning calorimetry (DSC), and molecular properties-size exclusion chromatography-multi-angle light scattering-differential refractive index (SEC-MALS-DRI) to ensure the quality of the production process.

Fig.2 The flowchart of the CDTase treatment. (CD BioGlyco)

Applications

• CDTase-treated starch produces coupling sugar with refractory properties.
• CDTase-treated starch can be used in the paper industry for surface gluing and coating.
• It uses trans glycoside viability prepared clade oligosaccharides.
• Adding CDTase-treated starch in the paper industry improved the writing performance of paper.

Advantages

• Product stability was significantly increased.
• CDTase-treated starch is less susceptible to alpha-amylase hydrolysis.

CD BioGlyco has many years of successful experience in the field of Modified Starch Manufacture. We also provide efficient Physical Modification, Chemical Modification, Enzymatic Modification, and Genetic Modification services. We have always been well received by customers with quality service and reliable products. If you would like to learn more about the manufacturing of starch, please do not hesitate to contact us.

• Amylomaltase Treatment