Where Is Oat Beta-Glucan?

Oat is the richest and most economical source of water-soluble beta-(1,3/1,4)-glucan, which exists in the cell wall of oat endosperm as dietary fiber and makes up about 70% of all components.

Fig.1 Beta-glucan in the cell wall of oat endosperm stained with calcofluor. (Wang & Ellis, 2014)

Advantages of Oat Beta-Glucan

Oat is a food ingredient that is often seen as healthier and more nutritious than other major grains. Oat beta-glucan acts as a functional ingredient that thickens and increases stability in the emulsion, giving the food a creamy taste. In addition, it has superior pharmaceutical value in helping to maintain healthy cholesterol by removing pre-existing cholesterol in the body, reducing dietary cholesterol intake, binding to saturated fat and trans fatty acids, and blocking cholesterol production through the effect of the gut microbiota. It can maintain normal blood glucan levels as a low glycemic index food, slow the release of saccharides in the digestive tract, and prevent diabetes.

Oat Beta-Glucan Manufacture Service

CD BioGlyco integrates efficient and advanced oat beta-glucan separation and extraction processes. We provide customers with professional oat beta-glucan manufacture technologies.

Fig.2 Personalized oat beta-glucan service. (CD BioGlyco)

• Conventional methodologies
• Acidic Extraction: Oat flour→reflux with ethanol→mix with sodium hydroxide and stir on a hot plate with a magnetic stirrer→centrifuge and gather the supernatant→adjust pH with acid→centrifuge and mix supernatant with ethanol→centrifuge and gather sediment→dry pellets.
• Alkaline Extraction: Be similar to the acidic extraction except for adjusting pH with alkali.
• Enzymatic Extraction: Be similar to the acidic extraction except for adjusting the supernatant at pH 7 and treating it with α-amylase and protease enzyme.
• Optimized methodologies
• Ammonium sulfate fractional precipitation: After dissolved in water, oats are precipitated in an ammonium sulfate solution. The purity of the product can be above 98%.
• Ethanol and enzyme-hot water two-step process: The molecular integrity and high viscosity of beta-glucan can be maintained effectively by the method.
• Advanced technologies
• Ultrasonic Assisted Extraction: The method promotes the release of geta-glucan mainly by mechanical effect and cavitation of ultrasonic wave. It has the advantages of high polysaccharide yield and fast extraction speed.
• Subcritical-water extraction: It is a new single-step process for beta-glucan extraction under high temperatures and high pressure. The extraction time will be greatly shortened and the yield will be more than triple.
• Combined methodologies
• Isoelectric precipitation combined with ethanol precipitation: The method has the advantages of simple operation, and is easily applied in industrial production.
• Ethanol precipitation combined with polyamide column chromatography: The separation effect is excellent, and it is suitable for products in small amounts but high purity.

Besides various conventional and advanced extraction techniques, CD BioGlyco also prepared kinds of polysaccharide purification services for clients. We select the most suitable extraction and purification methodologies according to your specific requirements, guaranteeing that you get access to the best oat beta-glucan product in the most economical way.

Applications

• Functional ingredients of food
• Pharmaceutical development: lower cholesterol and blood glucan levels
• Prevention of oral diseases
• Improve intestinal flora

Advantages

• Optional and customized extraction and separation processes
• Application of advanced technology
• High-quality product

CD BioGlyco has advanced production equipment and well-trained researchers in oat-beta glucan manufacture. Our intimate service has been consistently praised around the world. We also provide other Beta-Glucan Manufacture Services. Relative Quantification Service, Structural Analysis Service, Fermentation Profiling Service, and Vaccine Adjuvant Development Service are accessed. If you are interested in our services, please contact us for more details.

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