Properties of Endoamylse Treatment

Amylase is a class of enzymes that hydrolyzes α, 1-4 glycosidic bonds and α, 1-6 glycosidic bonds to obtain products for instance glucose, oligosaccharide, or dextrin. According to the different hydrolysis methods, it is divided into endocytic amylase and Exoamylase. Enolytic amylase directly hydrolyzes random glycosidic bonds within the reducing end of the polysaccharide chain; any α,1-4 glycosidic bonds inside the starch molecule as the hydrolysis object, so the hydrolysis products are not a single component, but the final hydrolysis products often contain glucose. The catalytic mechanism of the endoamylases family retains double displacement including the two catalytic residues in the active site, glutamate as an acid/base catalyst and aspartate as a nucleophile. With starch as raw material and with the aid of enzymes, the hydrolysis yields fructose, maltodextrin, glucose, and related derivatives, which better expands the application of starch.

Fig.1 The catalytic mechanism of the amylase family. (Marc, et al., 2002)

Endoamylse Treatment Services

The most important class of enzymes of endoamylse is α-Amylase. CD BioGlyco not only provides endoamylase ingredients but also has a complete process to apply enzymes to cereal starch. Due to the structural diversity of endoamylse and starch, their combination, detection, and target analysis is a challenging task. At present, there are few reports on the changes in starch structure after enzyme modification, so the enzyme modification process of starch granules is not clear, and the quantitative method of the degree of starch granule enzyme modification is lacking. At CD BioGlyco, We have a rapid quantification method for studying the enzymatic modification of starch granules, After incubation with endonuclease, the morphology was observed by polarizing microscopy and scanning electron microscope (SEM), and relevant indicators of modified starch were detected by kit and high performance liquid chromatography (HPLC). This method is fast to analyze and monitor product changes in real-time, and HPLC has the advantage of good reproducibility, which is important for promoting starch processing and application.

Fig.2 Starch enzymatic modification analysis flowchart. (CD BioGlyco)

Applications

• Industrial production of glucose and fructose from starch
• Added to the detergent to improve the decontamination capacity
• Production of sugar syrup from liquefied grain starch

Advantages

• The unique process allows the enzyme to remain active at 90 degrees Celsius
• The structural particles are not destroyed in the modification process

CD BioGlyco is a leader in carbohydrate manufacture and has many methods available for Enzyme Modification analysis. We have constantly optimized processes to modify the high-quality starch from the source to ensure the quality of the product, I believe that we will be your best partner in the Carbohydrate Manufacture road. If you are interested in our services, please feel free to contact us for more details.

• Exoamylase Treatment
• Debranching Enzyme Treatment
• Transferase Treatment
• α-Amylase Treatment