Types of Kestoses

Kestoses belong to the class of fructooligosaccharides. Kestoses are classified into 3 main groups according to their structure: 1-kestose, 6-kestose, and neokestose. 1-Kestose possesses D-fructofuranosyl units linked by β-(2→1) bonds. 6-Kestose involves β-(2→6) linkages connecting the fructosyl units. Moreover, neokestose features a fructosyl unit connected to the glucose component of the terminal sucrose via a β-(2→6) bond. It is not as commonly found in the natural environment. The differences in their chemical structures are vital for their biological functions. The structures of those kestoses are shown in the following picture. One of the most prevalent types is 1-kestose, which is abundant in numerous plant species. It is shown that 1-kestose has the potential to prevent bowel movement failure and high blood lipid levels. As a consequence, it is important to produce kestose.

Fig.1 The Structures of kestoses. (CD BioGlyco)

Kestose Manufacture Service at CD BioGlyco

CD BioGlyco utilizes Enzymatic Process for the manufacture of 1-kestose. To be specific, we purify, and characterize the sucrose 1-fructosyltransferase from Aspergillus foetidus. The enzyme demonstrates a high level of efficiency in converting sucrose to 1-kestose, while it does not result in the formation of fructans with higher polymerization degrees. Then we clone and sequence the cDNA that encodes the sucrose 1-fructosyltransferase from A. foetidus. Subsequently, we expressed it heterologously in Saccharomyces cerevisiaehas due to its competence for protein glycosylation. We purify the sucrose 1-fructosyltransferase produced by S. cerevisiaehas by utilizing ammonium sulfate precipitate, anion-exchange chromatography, and size exclusion chromatography.

Fig.2 The production process of 1-kestoses. (CD BioGlyco)

Besides, CD BioGlyco also provides 6-kestose manufacture service by β-fructofuranosidase from Schwanniomyces occidentalis. The β-fructofuranosidase from Sw. occidentalis yields a higher amount of 6-kestose both at its kinetic maximum and at the point of reaction equilibrium. Before employing the enzyme, we conduct a biochemical characterization of β-fructofuranosidase activity, which involved enzyme purification and evaluation of substrate specificity and kinetic properties. Then we employ the gradient method to purify 6-kestose. To guarantee the quality, we utilize high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR) to analyze 6-kestose.

Fig.3 The production process of 6-kestoses. (CD BioGlyco)

In addition, CD BioGlyco offers neokestose production service by using Phaffia rhodozyma. We employ a sophisticated entrapment-encapsulation technique, specifically the chitosan-coated alginate method, to immobilize P. rhodozyma. Because immobilization continues to offer advantages such as providing protection to the yeast from potentially harmful reaction environments and enhancing overall cell stability. Besides, the neokestose concentration and β-fructofuranosidase activity could be improved by optimizing the conditions for immobilized cells. Finally, HPLC and NMR are used to analyze neokestose.

Fig.4 The production process of neokestoses. (CD BioGlyco)

Applications

• Kestose can be used as a prebiotic ingredient in functional foods and beverages to promote the growth of beneficial gut bacteria and improve digestive health.
• Kestose can used in the food industry to improve the nutrition of food and the human diet.

Advantages of Us

• We possess the ability to produce any sugars you want.
• We use diverse technologies for the analysis of products to ensure their quality.
• We have a classical research team to develop a proposal.

CD BioGlyco is skilled in kestose manufacture. With years of experience, we use our unrivaled expertise for carbohydrate production. Our research team professionally provides One-Stop Solution for Carbohydrate Manufacture for our clients. If our services resonate with your needs, we invite you to contact us promptly to gather more detailed insights.

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