Beta-Glucan Quantification Service at CD BioGlyco

Depending on the origin, β-glucans show differences in linkage, branching, length of side chains, and molecular weight. Thus, beta-glucan exhibits diversity in its structure and functionality. It is very necessary to quantify beta-glucan. At CD BioGlyco, we provide a variety of methods for the quantitative analysis of beta-glucan. These methods provide us with accurate and reliable quantitative tools. Our researchers provide the best experimental design according to the research needs of our customers. We offer the following quantitative analysis services:

• Colorimetry method

Commonly used methods are the phenol sulfuric acid method, anthrone sulfuric acid method, and lichenous sulfuric acid method, etc. After treatment, the ultraviolet spectrophotometer is used for measurement, within a certain range, and the absorbance and sugar content have a linear relationship. We provide detection, curve plotting, data analysis, and other services.

• Chromatography method
• Gas chromatography-mass spectrometry (GC-MS)

Based on basic detection, with simple saccharide residue type, bond ligation method, sugar ring form, polymerization degree, and other relevant information after combining with mass spectrometry, our company adopts pre-derivatization, capillary chromatography column, and programmed heating to provide customers efficient and comprehensive quantitative services.

• High-performance liquid chromatography (HPLC)

We provide visible light detection methods and ultraviolet derivatization in two ways. According to your product characteristics, we provide several detectors and derivatization methods, divided into pre-column derivatization and post-column derivatization. Detector types of choices are also more, differential detectors and ultraviolet detector variety. This method has high sensitivity, good repeatability, and small error, to provide you with the best quality product quantitative services.

• Thin-layer chromatograph (TLC)

We use TLC to perform quantitative comparative analysis in the presence of the standard, which has a wide linear range and is sensitive and rapid.

• Near-infrared (NIR) spectroscopy model

We used Fourier transform near-infrared diffuse reflectance spectroscopy to quickly discriminate, and the polysaccharide quantitative analysis model has certain versatility in terms of prediction accuracy, stability, and adaptability.

• Other methods

Moreover, our company provides Congo red assay for the quantitative determination of dextran with a β-triple helix structure. This assay specifically binds to polysaccharides with a triple helix structure, providing you with a specific and efficient quantitative assay. We also provide the quantification of polysaccharides using nuclear magnetic resonance (NMR), NIR, Kit, and other methods, and have successfully provided analysis for many customer services.

Fig.1 Graphical representation of beta-glucan quantification services. (CD BioGlyco)

Advantages

• Beta-glucan quantification with high sensitivity and high throughput
• A first-class customized technical service team

CD BioGlyco has developed a variety of reliable and sensitive beta-glucan quantitative strategies to help researchers better understand complex Polysaccharides. Our high-quality scientific services will greatly improve your research efficiency and accelerate research progress. If you need Beta-Glucan manufacture, Structural Analysis, Fermentation Profiling, or Vaccine Adjuvant Development services, please contact us for further assistance.

• Beta-Glucan Manufacture Service
• Beta-Glucan Structural Analysis Service
• Beta-Glucan Fermentation Profiling Service
• Beta-Glucan Vaccine Adjuvant Development