The Principle of Chemical Hydrolysis

Acid hydrolysis is the main treatment before enzymatic hydrolysis of lignocellulosic biomass. Generally, specialized chemical degradation is performed under acidic conditions to obtain glucose, xylose, furan derivatives or levulinic acid, etc. For polysaccharides that are difficult to hydrolyze due to their rigid crystalline structure, hydrolysis is driven by concentrated acid or supported catalyst acid. In addition, the technology to produce oligosaccharides by acid hydrolysis in a controlled process has been advancing.

Fig.1 Production of reducing agaro-oligosaccharides through partial acid hydrolysis of agarose. (Ducatti, et al., 2011)

Chemical Hydrolysis Services at CD BioGlyco

As an outstanding company in the field of glycoscience, CD BioGlyco provides clients with high-quality One-Stop Solutions for Carbohydrate Manufacture. We provide advanced manufacturing services for oligosaccharides such as xylooligosaccharides, pectin-oligosaccharides, galactooligosaccharides, agar-oligosaccharides, carra-oligosaccharides, and cello-oligosaccharides using chemical hydrolysis technology.

CD BioGlyco provides a comprehensive Oligosaccharide Manufacture Service that involves the production of galactan oligosaccharides through partial acid hydrolysis. This process allows to obtaining of stable reducing neutral and acidic (sulfate) oligosaccharides with 3,6-anhydrogalactose at the reducing end. By applying partial acid hydrolysis to agarose and kappa-carrageenan, we specifically cleave the 3,6-anhydro-galactosidic linkages, resulting in reducing di- or tetrasaccharides that contain hydrated aldehydes as terminal units.

To address the challenge posed by cellulose's rigid crystalline structure, we offer acid hydrolysis of carbon-supported catalysts to produce oligomeric cellulose. Carbon materials with oxygen-containing functional groups exhibit potential as heterogeneous catalysts for synthesizing cellooligosaccharides from cellulose. These functional groups remain stable under the required ball milling and hydrothermal conditions for cellulose hydrolysis. The reaction involves the adsorption of cellulose onto the carbon surface through CH-π and hydrophobic interactions, followed by the hydrolysis of β-1,4-glycosidic bonds by acidic functional groups present on the catalyst surface. In a semi-flow reactor, cellulose is co-milled and hydrolyzed using this microporous catalyst, resulting in high yields of cellooligosaccharides. The adsorption of cellulose on the catalyst surface during the process helps prevent further degradation. Alternatively, chemically homogeneous catalysts such as H₃PO₄ dissolve and hydrolyze cellulose, yielding high yields of cello-oligosaccharides.

Fig.2 Oligosaccharides produced by chemical hydrolysis. (CD BioGlyco)

Applications

• Chemical hydrolysis as a production technique produces oligosaccharides with promising applications in the food and agricultural industries.
• Acid hydrolysis in a controlled process to produce high yields of oligosaccharides is an advanced production technique.
• Chemical hydrolysis is used as the main treatment step before enzymatic hydrolysis.

Advantages

• Acidic hydrolysis with carbon-supported catalysts hydrolyzes rigid crystalline polysaccharides.
• A chemically homogeneous catalyst dissolves and hydrolyzes polysaccharides, yielding high yields of oligosaccharides.
• Controlled chemical hydrolysis is expected to hydrolyze polysaccharides that cannot be hydrolyzed under normal regulation.

CD BioGlyco has first-class technology and well-trained technicians. Our dedicated team of experts works tirelessly to provide Physical-/Chemical Process for Oligosaccharide Manufacture that meets clients' needs and requirements. If you need more details, please don't hesitate to contact us.

• Supercritical Treatment
• Chemical Synthesis