Current Research Status of Oligosaccharide Manufacture

Oligosaccharides are comprised of 2-10 monosaccharide molecules with sweet flavor but low calories. Due to their low impact on blood glucose and insulin, they are widely used in food, beverages as well and pharmaceuticals. Common methods used in oligosaccharide manufacture include enzymatic synthesis, chemical synthesis, and extraction from natural sources. Enzymatic synthesis utilizes specific enzyme-catalyzed reactions to efficiently synthesize it under suitable substrate conditions. Different types of oligosaccharides can be synthesized by adjusting the substrate composition and enzyme specificity. The construction of oligosaccharide structures by chemical synthesis requires the synthesis of complex glycosyl synthons followed by chemical reactions to generate the target oligosaccharides. Some oligosaccharides can be derived from natural ingredients, such as xylitol and isomaltulose. Current research focuses on developing efficient and economically viable oligosaccharide production technologies and exploring the application areas of oligosaccharides. 

Fig.1 Structure of some typical human milk oligosaccharides. (Palur, et al., 2023)

Microbial Purification/Selective Fermentation for High-Purity Oligosaccharide Manufacture at CD BioGlyco

At CD BioGlyco, we use a variety of advanced Technologies to provide you with professional Oligosaccharide Manufacturing services. Our team of experts carefully selects and cultivates microbial strains optimized for oligosaccharide synthesis, maximizing yield and efficiency while minimizing unwanted by-products. The services we provide include but are not limited to the following:

• Microbial purification for high-purity oligosaccharide manufacture

We use cell harvesting and cell crushing to release the oligosaccharides. Then we promote oligosaccharide manufacturing by preferring high-yielding strains and utilizing fermentation culture. Cell harvesting and cell crushing are used to release the oligosaccharides. Subsequently, we remove solid particles and impurities by filtration and separation, followed by concentration and purification to remove water. After chromatography to remove impurities, the final step is crystallization to obtain a high-purity oligosaccharide product.

• Selective fermentation for high-purity oligosaccharide manufacture

We optimize strains and fermentation conditions along with effective bioprocess control. This includes monitoring and control of the fermentation process, such as real-time monitoring of bacterial growth, metabolite concentration, etc., and timely adjustment of culture conditions to ensure high yield and purity of the target oligosaccharides.

We adjust and optimize the experimental steps according to the specific situation and your requirements for experimental details to achieve the manufacture of high-purity oligosaccharides.

Fig.2 Experimental procedure of microbial purification. (CD BioGlyco)

Applications

• Certain oligosaccharides can be used as carriers for drugs to help deliver them to specific cells or tissues.
• Oligosaccharides can be used in the preparation of drugs such as vaccines and antibiotics.
• Oligosaccharides have important applications in biotechnology. They can be used as a source of energy and carbon in culture media to facilitate microbial fermentation processes.

Advantages of Us

• Microbial purification and selective fermentation techniques allow precise control of reaction conditions for the selection of oligosaccharide-manufacture strains and metabolic pathways. This allows for the optimization of oligosaccharide manufacture and structure to meet specific requirements and goals.
• We have mature technology and specialized laboratory technicians. 
• We design suitable microbial purification and selective fermentation processes for you based on the type, structure, and size of the oligosaccharides and optimize the reaction parameters to meet your requirements.

At CD BioGlyco, we prioritize quality and client satisfaction. We are committed to providing our clients with high-quality microbial purification/selective fermentation for high-purity oligosaccharide manufacture. If you are interested in our services, please contact us for more details. We look forward to being your competent assistant in your research.

• Batch Chromatography for High-Purity Oligosaccharide Manufacture
• Simulated Moving Bed (SMB) Chromatography for High-Purity Oligosaccharide Manufacture