Applications of Gel Column Chromatography (GCC)

GCC is an effective technique for the purification and separation of different types of biopolysaccharides. By selecting a suitable gel column and buffer system, precise separation of biopolysaccharides in the solution can be performed based on the molecular weight, branching structure, or other characteristics of the biopolysaccharides. In addition, GCC can be used to remove reactants or impurities from the sample to further improve the purity and quality of polysaccharides. GCC features the advantages of good selectivity, easy operation, and excellent separation effect, and applies to the purification of various types of biopolysaccharides, such as polysaccharide drugs, natural polysaccharides, and so on. It provides researchers with a reliable means for obtaining biopolysaccharide samples with high purity and good activity, a powerful tool for biopolysaccharide purification, and an important basis for in-depth research and application of biopolysaccharides. It helps researchers obtain high-purity biopolysaccharide samples, thus promoting the development of the field of glycobiology.

Fig.1 Gel-permeation chromatography. (Coskun, 2016)

GCC-based Polysaccharide Purification Service at CD BioGlyco

GCC is extensively utilized in the fields of biochemistry, molecular biology, and pharmaceuticals. GCC allows obtaining high-purity samples from complex mixtures for further study of their structure and function. CD BioGlyco is a company specializing in Polysaccharide Manufacturing, especially Starch Manufacturing and Glucan Manufacturing. We also provide professional GCC-based Polysaccharide Purification Services to help clients accelerate their research process in glycobiology.

First, we select the proper gel material and choose the appropriate pore size according to the size of the target molecule. Pre-equilibrate the gel column and buffer to ensure that the pores inside the gel column are fully saturated and air bubbles are removed. Then the polysaccharide sample is dissolved to be purified in the appropriate buffer and slowly added to the top of the gel column. We use a buffer to elute the column, adjusting the buffer composition and flow rate as needed. After that, we collect different separated fractions depending on the separation effect and retention time. The absorbance or fluorescence emission is monitored in real time using a chromatographic detector to determine the location of the target fraction. Finally, we detect the structure by using analytical instruments such as mass spectrometry, the purified target components are concentrated and dried to obtain a pure target product.

Fig.2 Flowchart of GCC-based polysaccharide purification. (CD BioGlyco)

Applications

• Polysaccharides are used in the preparation of products such as creams, lotions, and masks due to their moisturizing, softening, antioxidant, and fiber-strengthening properties.
• Polysaccharides play a crucial role as versatile additives in both water treatment and soil remediation applications.  
• In the field of biosensor technology, these natural polysaccharides can be utilized to create biosensors that offer highly sensitive and selective detection of a wide range of biomolecules and environmental pollutants.

Advantages of Us

• We use high-quality instrumentation and software systems that allow real-time monitoring of sample separation and purification, as well as real-time analysis and review of data.
• To ensure the quality of our products, we strictly follow the requirements of international standards and take a series of measures for quality control and quality management.
• Our management team consists of a group of experienced professionals with global business backgrounds. They have extensive experience in working and managing multinational corporations and are always aware of the latest and most up-to-date developments in the industry.

CD BioGlyco offers comprehensive support and expertise in the development of glycobiology research. Our highly skilled and dedicated technicians will provide you with the most appropriate GCC-based polysaccharide purification solution. If you are interested in our services, please feel free to contact us for more details.

• Ion Exchange Column Chromatography-based Polysaccharide Purification Service
• Affinity Chromatography-based Polysaccharide Purification Service